水生生物学报
水生生物學報
수생생물학보
ACTA HYDROBIOLOGICA SINICA
2001年
2期
191-194
,共4页
安利国%孟广勋%杨桂文%尹苗%王钦东
安利國%孟廣勛%楊桂文%尹苗%王欽東
안리국%맹엄훈%양계문%윤묘%왕흠동
鲤%发育%粘液细胞
鯉%髮育%粘液細胞
리%발육%점액세포
Carp (Cyprinus carpio L.)%Development%Mucous cell
利用酸性条件下的阿新蓝染色和过碘酸-雪夫氏试剂反应相接合的方法(AB-PAS染色),对鲤早期发育过程中粘液细胞的变化作了初步研究。结果表明,鲤的粘液细胞在受精卵孵化前一天出现,密度随个体的发育而增大,成分随发育过程的进行而趋于复杂,鲤的粘液细胞可分为四种类型,Ⅰ型和Ⅱ型含单一成分,为幼稚型,Ⅲ型和Ⅳ型含复合成分,为成熟型,鲤粘液细胞在不同组织器官中的分布是不均匀的,其主要分布区是皮肤、口腔、鳃、消化道等部位。
利用痠性條件下的阿新藍染色和過碘痠-雪伕氏試劑反應相接閤的方法(AB-PAS染色),對鯉早期髮育過程中粘液細胞的變化作瞭初步研究。結果錶明,鯉的粘液細胞在受精卵孵化前一天齣現,密度隨箇體的髮育而增大,成分隨髮育過程的進行而趨于複雜,鯉的粘液細胞可分為四種類型,Ⅰ型和Ⅱ型含單一成分,為幼稚型,Ⅲ型和Ⅳ型含複閤成分,為成熟型,鯉粘液細胞在不同組織器官中的分佈是不均勻的,其主要分佈區是皮膚、口腔、鰓、消化道等部位。
이용산성조건하적아신람염색화과전산-설부씨시제반응상접합적방법(AB-PAS염색),대리조기발육과정중점액세포적변화작료초보연구。결과표명,리적점액세포재수정란부화전일천출현,밀도수개체적발육이증대,성분수발육과정적진행이추우복잡,리적점액세포가분위사충류형,Ⅰ형화Ⅱ형함단일성분,위유치형,Ⅲ형화Ⅳ형함복합성분,위성숙형,리점액세포재불동조직기관중적분포시불균균적,기주요분포구시피부、구강、새、소화도등부위。
In this paper, changes of mucous cells in the early developmentalstage of carp, Cyprinus carpio, were studied by the method of histochemical techniques-a combination of Alcine Blue staining under acid conditions and PAS reaction. The mucous cells were first observed in hatching glands at the 1st day before hatching. They appeared immediately in the mouth cavity, pharynx, gills and gut after hatching. With the development of the carp, the density, type and content of mucous cells increased and became complicated. Type Ⅰ and Type Ⅳ of mucous cells containing unitary mucoitin are in immature stage and appeared earlier than Type Ⅱ and Type Ⅲ which are in mature stage and contain multiple mucoitins. The mucous cells mainly distributed in mucousal epidermis of such organs as skin、mouth、gill and intestine.