CAJ | 학술논문

背景:单胺类递质参与机体多种功能活动的调节和某些病理生理过程,但有关低功率激光对单胺递质的影响报道甚少,而且结果很不一致,甚至矛盾.目的:探讨氦氖激光对小鼠脑组织多巴胺、5-羟色胺有无影响.设计:完全随机的实验研究.地点和材料:北京大学第一医院中心实验室,材料为昆明种健康雄性小鼠90只,由北京大学动物中心提供,体质量22～27 g.干预:采用随机数字表将小鼠单纯随机分为5组:正常对照组,假照射组,激光照射A组,激光照射B组,激光照射C组.在麻醉状态下,切开小鼠头部皮肤,暴露颅骨及脑组织,用氦氖激光仪垂直照射小鼠额叶皮质,立即断颈杀死,取其全脑,制备生物样品.主要观察指标:观察激光照射后脑组织多巴胺、5-羟色胺含量的变化,采用高效液相色谱-电化学检测方法测定.结果:多巴胺含量(ng/g):假照射组:446.5±7.1;激光照射A组:485.4±10.1;激光照射B组:505.8±10.3激光照射C组:524.1±10.5;激光照射A,B,C组分别与假照射组比较,差异均有统计学意义(A组P＜0.05,F=4.64,B组P＜0.01,F=8.63;C组:P＜0.01,F=14.52),血清素含量(ng/g):假照射组:1 198.0±28.5;激光照射A组:1 283.8±25.1;激光照射B组:1 337.0±26.8;激光照射C组:1 429.9±30.1;激光照射各组与假照射组比较,差异均有统计学意义(A组P＜0.05,F=4.92,B组P＜0 01,F=9.75;C组P＜0.01,F=16.83),激光照射各组可明显增加小鼠脑组织多巴胺、5-羟色胺含量.结论:氦氖激光脑局部照射可增加小鼠脑组织多巴胺、5-羟色胺含量,且与激光剂量有关.
배경:단알류체질삼여궤체다충공능활동적조절화모사병리생리과정,단유관저공솔격광대단알체질적영향보도심소,이차결과흔불일치,심지모순.목적:탐토양내격광대소서뇌조직다파알、5-간색알유무영향.설계:완전수궤적실험연구.지점화재료:북경대학제일의원중심실험실,재료위곤명충건강웅성소서90지,유북경대학동물중심제공,체질량22～27 g.간예:채용수궤수자표장소서단순수궤분위5조:정상대조조,가조사조,격광조사A조,격광조사B조,격광조사C조.재마취상태하,절개소서두부피부,폭로로골급뇌조직,용양내격광의수직조사소서액협피질,립즉단경살사,취기전뇌,제비생물양품.주요관찰지표:관찰격광조사후뇌조직다파알、5-간색알함량적변화,채용고효액상색보-전화학검측방법측정.결과:다파알함량(ng/g):가조사조:446.5±7.1;격광조사A조:485.4±10.1;격광조사B조:505.8±10.3격광조사C조:524.1±10.5;격광조사A,B,C조분별여가조사조비교,차이균유통계학의의(A조P＜0.05,F=4.64,B조P＜0.01,F=8.63;C조:P＜0.01,F=14.52),혈청소함량(ng/g):가조사조:1 198.0±28.5;격광조사A조:1 283.8±25.1;격광조사B조:1 337.0±26.8;격광조사C조:1 429.9±30.1;격광조사각조여가조사조비교,차이균유통계학의의(A조P＜0.05,F=4.92,B조P＜0 01,F=9.75;C조P＜0.01,F=16.83),격광조사각조가명현증가소서뇌조직다파알、5-간색알함량.결론:양내격광뇌국부조사가증가소서뇌조직다파알、5-간색알함량,차여격광제량유관.
BACKGROUND: It is known that monoamine may take part in the regulation of various functional activities of the body and also in the pathophysiological processes, but the reports about the influnences of low-power laser radiation over monoamine are still rare. Moreover, the results gained are very inconsistent, and some are even contradictory to each other.OBJECTIVE: To elucidate whether low power He-Ne laser radiation can affects serotonin(5-HT) and dopamine(DA) in brain.DESIGN: A completely randomlized study based on the experimental.SETTING AND MATERIALS: The experiment was done in the Central Laboratory of the First Hospital of Beijing University. Materials used were 90Kunming male mice with body-weight 20 -27 g, supplied by Beijing University Animal Centre.INTERVENTIONS: The mice were randomly assigned to 5 groups with randomized arithmetic form. They are normal control group, sham radiation group, aser irradiation group A(13.8 J/cm2), aser irradiation group B (27.6 J/cm2) and laser irradiation group C( 55.1 J/cm2) . The cranial bone and brain of the mouse was exposed under mild anesthesia and irradiated on the sinciput with different doses of He-Ne laser. Then the mice were killed by decapitation. Living creature samples were made.MIAN OUTCOME MEASURES: The cerebral serotonin and dopamine were observed and measured by means of high performance liguid chromatography-electrochemistry ( HPLC-EC ).RESULTS: Dopamine contents(ng/g) were 485.4 + 10. 1 in laser group A,505.8 + 10. 3 in laser group B and 524. 1 ± 10.5 in laser group C, with significant difference as compared with that of the sham laser group(446.5 +7.1)(P ＜0.05, F=4.64; P ＜0.01, F=8.63;P ＜0.01, F=14.52, respectively) . Serotonin contents(ng/g) were 1 283.8 +25.1 in laser group A, 1 337.0 ± 26. 8 in laser group B and 1 429.9 ± 30. 1 in laser group C, significantly higher than that of the sham laser group(1 198.0 ±28.5 )(P ＜0.05, F=4.92; P ＜0.01, F=9.75; P ＜0.01, F=16. 83, respectively). It was found that there was a significant increase in the cerebral serotonin and dopamine in the irradiated mice, and within the laser dose range used, the larger is the laser dose was, the more DA and 5-HT are.CONCLUSION: He-Ne laser radiation may cause a significant increase in 5-HT and DA in the brain of the mice, and the increase is related to the dose of laser radiation.