CAJ | 학술논문

以百合新鲜鳞叶为材料,利用RAPD分子标记技术对16份不同生态型的百合种质的遗传多样性和亲缘关系进行了分析.研究结果表明,从120条随机引物中筛选出35条有效引物,共得到769个扩增位点,其中628个位点具有多态性,占81.7%.UPGMA聚类分析结果表明.16份百合种质在阀值为0.9407处分为2个聚类群,即百合科两个不同属的植物:百合属和大百合属;阈值为0.5790处,百合属被分为3个不同的种:百合、卷丹和细叶百合.聚类结果和亲缘关系分析表明不同供试百合之间的遗传多样性较高,亲缘关系较远,且各种质遗传距离与地理距离具有一定的相关性.
이백합신선린협위재료,이용RAPD분자표기기술대16빈불동생태형적백합충질적유전다양성화친연관계진행료분석.연구결과표명,종120조수궤인물중사선출35조유효인물,공득도769개확증위점,기중628개위점구유다태성,점81.7%.UPGMA취류분석결과표명.16빈백합충질재벌치위0.9407처분위2개취류군,즉백합과량개불동속적식물:백합속화대백합속;역치위0.5790처,백합속피분위3개불동적충:백합、권단화세협백합.취류결과화친연관계분석표명불동공시백합지간적유전다양성교고,친연관계교원,차각충질유전거리여지리거리구유일정적상관성.
In this study, the genetic diversity and phylogenetic relationship among 16 genotypes of Lily collected from different ecological sources was analyzed by RAPD. Thirty-five available RAPD primers were screened from 120 random primers, and a total of 769 DNA bands were amplified, in which 628 bands were polymorphic, the percentage is 81.7%. Cluster analysis showed that the 16 Lily germplasms were divided into two groups at 0.9407. Corresponding to two genus in liliaceae: Lilium and Cardiocrinum, and that germplasms in Lilium were further divided into three different types at 0.5790: Liliam brownii F.E. Brown var.viridulum Baker, L. lancifotium Thunb and L. pumilum DC. Results suggested that the genetic diversity is very high among different Lily germplasms and the genetic distance among germplasms was relative to their geographic distance.