CAJ | 학술논문

目的:构建冷诱导型启动子CBF 3基因的植物表达载体,并将其转入烟草.方法:以拟南芥基因组DNA为模板,通过特异PCR扩增,克隆冷诱导表达启动子CBF 3(C-repeat bindingfactor).用CBF 3启动子替换pBI121载体上的35S启动子构建新的载体pBC-GUS,通过农杆菌介导的叶盘法转化烟草.结果:获得了转基因烟草,转基因烟草的GUS组织化学染色及PCR分析结果表明,在低温诱导下,CBF 3启动子可增强GUS基因表达.结论:CBF 3启动子可应用于植物抗冷基因工程研究.
목적:구건랭유도형계동자CBF 3기인적식물표체재체,병장기전입연초.방법:이의남개기인조DNA위모판,통과특이PCR확증,극륭랭유도표체계동자CBF 3(C-repeat bindingfactor).용CBF 3계동자체환pBI121재체상적35S계동자구건신적재체pBC-GUS,통과농간균개도적협반법전화연초.결과:획득료전기인연초,전기인연초적GUS조직화학염색급PCR분석결과표명,재저온유도하,CBF 3계동자가증강GUS기인표체.결론:CBF 3계동자가응용우식물항랭기인공정연구.
Objective: The expression vector of pBC - GUS was constructed and transferred into tobacco to get the transgenic plants. Method: Cold - induced promoter CBF 3(C - repeat binding factor) was amplified and cloned by PCR from the genomic DNA of the Arabidopsis thaliana. Promoter 35S of vector pBI121 was replaced by promoter CBF 3, 35S of vector pBI121 was replaced by promoter CBF 3, thus vector pBC - GUS was constructed and transferred into tobacco by co - culturing excised cotyledon explants with Agrobacterium tumefaciens, and transgenic plants were obtained. Result: PCR and GUS histochemical stain of transgenic tobacco showed that promoter CBF 3 induced by low temperature stress strengthened expression of gene GUS. Conclusion:Therefore, promoter CBF 3 could be used to plant gene engineering for cold resistance improvement.