CAJ | 학술논문

构建了基于多壁碳纳米管(Multi-walled carbon nanotubea,MWCNTs)和铂纳米颗粒(Pt-nano)的电流型L-乳酸生物传感器.将Sol-gel膜覆盖在L-乳酸氧化酶(L-lactate oxidase,LOD)和MWCNTs/Pt-nano修饰的电极表面.实验结果表明:传感器的最佳工作条件为:检测电压0.5 V;缓冲液pH 6.4;检测温度25℃.此传感器的响应时间为5 s,灵敏度是6.36μA/(mmol/L).连续检测4星期其活性仍保持90%,线性范围为0.2～2.0 mmol/L,且抗干扰能力强.在实际血样的检测中,此传感器与传统的分光光度法具有很好的一致性.
구건료기우다벽탄납미관(Multi-walled carbon nanotubea,MWCNTs)화박납미과립(Pt-nano)적전류형L-유산생물전감기.장Sol-gel막복개재L-유산양화매(L-lactate oxidase,LOD)화MWCNTs/Pt-nano수식적전겁표면.실험결과표명:전감기적최가공작조건위:검측전압0.5 V;완충액pH 6.4;검측온도25℃.차전감기적향응시간위5 s,령민도시6.36μA/(mmol/L).련속검측4성기기활성잉보지90%,선성범위위0.2～2.0 mmol/L,차항간우능력강.재실제혈양적검측중,차전감기여전통적분광광도법구유흔호적일치성.
An electrochemical L-lactate biosensor was fabricated by combining Platinum nanoparticles (Pt-nano) with multi-walled carbon nanotubes(MWCNTs).L-lactate oxidase(LOD) was immobilized on the surface of the glassy carbon electrode (GCE) modified with MWCNTs and Pt-nano.The surface of resulting LOD/MWCNTs/Pt-nano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD and to improve the anti-interference ability.The cyclic voltammetric results indicated that MWCNTs/Pt-nano catalyst displayed a higher performance than MWCNTs.Under the optimized conditions, i.e., applied potential of 0.5 V, pH 6.4, 25 ℃, the proposed biosensor's determination range was 0.2-2.0 mmol/L, response time was within 5 s, and the sensitivity was 6.36 (A/(mmol/L).It still kept 90% activity after 4 weeks.The fabricated biosensor had practically good selectivity against interferences.The results for whole blood samples analyzed by the present biosensor showed a good agreement with those analyzed by spectrophotometric method.