CAJ | 학술논문

利用微卫星标记技术,采用8对微卫星引物对凡纳滨对虾3个亲本群体(OI Q、SIS Q、Kona Bay Q)及其子一代(OI Z、SIS Z、Kona Bay Z)共计120个个体进行遗传分析.计算6个群体在8个微卫星位点上的平均等位基因数(Na)、平均有效等位基因数(Ne)、平均观测杂合度(Ho)、平均期望杂合度(He)、平均多态信息含量(PIC)和平均Hardy-Weinberg平衡指数(D),以上各参数均采用群体遗传学分析软件POPGENE 3.2处理,同时根据Nei的方法计算群体间的遗传距离和遗传相似系数.运用MEGA3.0软件,采取UPGMA方法根据6个群体的遗传距离进行聚类.结果表明:8对微卫星引物在6个群体中共检测到28个等位基因,每个位点的等位基因数为2～6个,平均等位基因数为 3.5;各位点的期望杂合度均比观测杂合度高;多态信息含量(PIC)值为0.479 4～0.769 9,说明这8个位点在凡纳滨对虾中具有较高的信息含量.在亲本和子代群体遗传结构分析中,子代的有效等位基因数、杂合度和多态信息含量等指标均略低于亲本群体,但子代的遗传多样性并未受到太大影响,仍保持较好的遗传力.
이용미위성표기기술,채용8대미위성인물대범납빈대하3개친본군체(OI Q、SIS Q、Kona Bay Q)급기자일대(OI Z、SIS Z、Kona Bay Z)공계120개개체진행유전분석.계산6개군체재8개미위성위점상적평균등위기인수(Na)、평균유효등위기인수(Ne)、평균관측잡합도(Ho)、평균기망잡합도(He)、평균다태신식함량(PIC)화평균Hardy-Weinberg평형지수(D),이상각삼수균채용군체유전학분석연건POPGENE 3.2처리,동시근거Nei적방법계산군체간적유전거리화유전상사계수.운용MEGA3.0연건,채취UPGMA방법근거6개군체적유전거리진행취류.결과표명:8대미위성인물재6개군체중공검측도28개등위기인,매개위점적등위기인수위2～6개,평균등위기인수위 3.5;각위점적기망잡합도균비관측잡합도고;다태신식함량(PIC)치위0.479 4～0.769 9,설명저8개위점재범납빈대하중구유교고적신식함량.재친본화자대군체유전결구분석중,자대적유효등위기인수、잡합도화다태신식함량등지표균략저우친본군체,단자대적유전다양성병미수도태대영향,잉보지교호적유전력.
Genetic variations of three species from primary parent (OI Q,SIS Q,Kona Bay Q) and their first filial generation (OI Z,SIS Z,Kona Bay Z) of Litopenaeus vannamei were detected using microsatellite marker technique.The amplified products of genomic DNA with 8 microsatellite makers indicated that 8 loci presents polymorphism,the number of total alleles were 28 in 6 species; the number of allele of every loci was 2 to 6; mean allele number (Na) was 3.5; expected heterozygosity value (He) were higher than observed heterozygosity value (Ho); mean polymorphism information content (PIC) ranged from 0.479 4 to 0.769 9,which showed these 8 loci posses relatively high information content.Through analysis of genetic structure in primary parent and their first filial,allele and effective allele number,observed heterozygosity and expected heterozygosity values and mean polymorphism information content (PIC) in filial generation were little lower than their primary parents.However,genetic diversity of filial generation is not influenced,and still maintains a good heritability.