中国行为医学科学
中國行為醫學科學
중국행위의학과학
2008年
7期
588-590
,共3页
王昌铭%孟雪%向静%程相铎%王景周%孙鸣%吉国泰
王昌銘%孟雪%嚮靜%程相鐸%王景週%孫鳴%吉國泰
왕창명%맹설%향정%정상탁%왕경주%손명%길국태
脐血单个核细胞%血管性痴呆%神经生长因子
臍血單箇覈細胞%血管性癡呆%神經生長因子
제혈단개핵세포%혈관성치태%신경생장인자
Human cord blood mononuclear cells%Vascular dementia%Nerve growth factor
目的 观察颈内动脉输注脐血单个核细胞(human cord blood mononuclear cells,HCMNCs)对血管性痴呆(vascular dementia,VD)大鼠认知功能及脑组织神经生长因子(nerve growth factor,NGF)含量的影响.方法 改良Pulsinellis四血管阻断法建立VD大鼠模型;体外分离HCMNCs,术后24 h颈内动脉输注数量为3×106/0.5ml的BrdU标记细胞;利用穿梭箱系统和ELISA法检测注射HCMNCs后2,4,8周VD大鼠学习记忆能力以及脑组织NGF含量的变化.结果 模型组大鼠AAR比率(55.4±4.5,42.1±4.5,44.2±3.6)明显低于对照组(91.7±3.9,90.0±4.3,92.5±5.0)(P<0.01),治疗组(67.1±3.3,69.2±4.7,70.8±4.7)较模型组显著提高(P<0.01).术后2周模型组大鼠脑组织NGF含量较对照组明显增高(P<0.01),4周时达到高峰(P<0.01),8周时则明显下降,与2周时相比差异有显著性(P<0.05);颈内动脉输注HCMNCs后治疗组大鼠脑组织NGF含量较模型组显著升高(P<0.01),4周时最高(P<0.01),8周时略有下降,但仍维持在较高水平,与4周时相比差异无显著性(P0.05).结论 颈内动脉输注HCMNCs可显著改善VD大鼠学习记忆能力,增加VD大鼠脑组织NGF含量,具有脑保护作用.
目的 觀察頸內動脈輸註臍血單箇覈細胞(human cord blood mononuclear cells,HCMNCs)對血管性癡呆(vascular dementia,VD)大鼠認知功能及腦組織神經生長因子(nerve growth factor,NGF)含量的影響.方法 改良Pulsinellis四血管阻斷法建立VD大鼠模型;體外分離HCMNCs,術後24 h頸內動脈輸註數量為3×106/0.5ml的BrdU標記細胞;利用穿梭箱繫統和ELISA法檢測註射HCMNCs後2,4,8週VD大鼠學習記憶能力以及腦組織NGF含量的變化.結果 模型組大鼠AAR比率(55.4±4.5,42.1±4.5,44.2±3.6)明顯低于對照組(91.7±3.9,90.0±4.3,92.5±5.0)(P<0.01),治療組(67.1±3.3,69.2±4.7,70.8±4.7)較模型組顯著提高(P<0.01).術後2週模型組大鼠腦組織NGF含量較對照組明顯增高(P<0.01),4週時達到高峰(P<0.01),8週時則明顯下降,與2週時相比差異有顯著性(P<0.05);頸內動脈輸註HCMNCs後治療組大鼠腦組織NGF含量較模型組顯著升高(P<0.01),4週時最高(P<0.01),8週時略有下降,但仍維持在較高水平,與4週時相比差異無顯著性(P0.05).結論 頸內動脈輸註HCMNCs可顯著改善VD大鼠學習記憶能力,增加VD大鼠腦組織NGF含量,具有腦保護作用.
목적 관찰경내동맥수주제혈단개핵세포(human cord blood mononuclear cells,HCMNCs)대혈관성치태(vascular dementia,VD)대서인지공능급뇌조직신경생장인자(nerve growth factor,NGF)함량적영향.방법 개량Pulsinellis사혈관조단법건립VD대서모형;체외분리HCMNCs,술후24 h경내동맥수주수량위3×106/0.5ml적BrdU표기세포;이용천사상계통화ELISA법검측주사HCMNCs후2,4,8주VD대서학습기억능력이급뇌조직NGF함량적변화.결과 모형조대서AAR비솔(55.4±4.5,42.1±4.5,44.2±3.6)명현저우대조조(91.7±3.9,90.0±4.3,92.5±5.0)(P<0.01),치료조(67.1±3.3,69.2±4.7,70.8±4.7)교모형조현저제고(P<0.01).술후2주모형조대서뇌조직NGF함량교대조조명현증고(P<0.01),4주시체도고봉(P<0.01),8주시칙명현하강,여2주시상비차이유현저성(P<0.05);경내동맥수주HCMNCs후치료조대서뇌조직NGF함량교모형조현저승고(P<0.01),4주시최고(P<0.01),8주시략유하강,단잉유지재교고수평,여4주시상비차이무현저성(P0.05).결론 경내동맥수주HCMNCs가현저개선VD대서학습기억능력,증가VD대서뇌조직NGF함량,구유뇌보호작용.
Objective To explore the effect of intracarotid administration of human cord blood mononuclear cells(HCMNCs) on cognitive function and contents of nerve growth factor(NGF) in the vascular dementia (VD) rats. Methods The VD rat model was established by modified pulsinellis 4-Vessel occlusion(4 VO). HCMNCs were isolated from human umbilical cord blood in vitro. The treatment group received intracarotid infusion 3×106/0.5 ml HCMNCs which were prelabelled with BrdU in vitro. The learning-memory abilities and the contents of NGF were observed at 2 w ,4 w and 8 w by computerized shuttle-training ease and Enzyme-linked immunoadsordent assay (ELISA). Results AAR ratio in the model group (55.4±4.5,42.1±4.5,44.2±3.6) showed significantly decrease compared with the control(91.7±3.9,90.0±4.3,92.5±5.0) (P<0.01), and the treatment(67.1±3.3,69.2±4.7,70.8±4.7) was significant higher than the model (P<0.01). The contents of NGF in the model group was significantly higher than that in the control at 2 w, increased to the highest level at 4 w, and then decreased at 8 w, but compared with that at 2 w, significant difference was found. Compared with the model group,the contents of NGF in the treatment showed significantly increase after infusion with HCMNCs, increased to the highest level at 4 w, and then decreased at 8 w but maintain the high level, there were no significant difference between that at 4 w and at 8 w. Conclusion Intraearotid administration of HCMNCs significantly improved the learning-memory abilities and increased the contents of NGF in the VD rats.
