中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
2期
213-217
,共5页
肺肿瘤%DNA双链断裂修复基因NBS1%基因多态%遗传易感性
肺腫瘤%DNA雙鏈斷裂脩複基因NBS1%基因多態%遺傳易感性
폐종류%DNA쌍련단렬수복기인NBS1%기인다태%유전역감성
Lung neoplasms%DNA double-strand break repair gene NBS1%Gene polymorphism%Genetic susceptibility
目的 探讨DNA双链断裂修复基因NBS1多态性与肺癌遗传易感性的关系.方法 采用病例对照设计,应用PCR-RFLP技术检测575例患者和575名对照的NBS1基因多态.结果 对照组和病例组NBS1 rs1805794的C/C、C/G、G/G基因型频率分别为25.9%、51.8%、22.3%和20.5%、52.3%、27.1%,两组分布差异有统计学意义(χ~2=6.38,P=0.04),携带C/G+G/G基因型个体患肺癌的风险是携带C/C基因型者的1.46倍(OR=1.46,95%C1:1.09～1.97).对照组和病例组NBS1 rs2735383的G/G、G/C、C/C基因型频率分别为37.9%、47.0%、15.1%和35.5%、48.5%、16.0%,两组分布差异无统计学意义(χ~2=0.75,P=0.69).携带Hap4-GC单体型或Hap4/Hap2单体型对者患肺癌的风险增加,OR值分别为1.70(95%CI:1.24～2.31)和1.75(95%CI:1.11～2.76),NBS1基因多态与吸烟有联合作用(P＜0.05).结论 NBS1 rs1805794 G/G基因型可能是肺癌的易感基因型,rs1805794和rs2735383位点构建的Hap4-GC单体型及Hap4/Hap2单体型对可能是肺癌的易感单体型和单体型对.
目的 探討DNA雙鏈斷裂脩複基因NBS1多態性與肺癌遺傳易感性的關繫.方法 採用病例對照設計,應用PCR-RFLP技術檢測575例患者和575名對照的NBS1基因多態.結果 對照組和病例組NBS1 rs1805794的C/C、C/G、G/G基因型頻率分彆為25.9%、51.8%、22.3%和20.5%、52.3%、27.1%,兩組分佈差異有統計學意義(χ~2=6.38,P=0.04),攜帶C/G+G/G基因型箇體患肺癌的風險是攜帶C/C基因型者的1.46倍(OR=1.46,95%C1:1.09～1.97).對照組和病例組NBS1 rs2735383的G/G、G/C、C/C基因型頻率分彆為37.9%、47.0%、15.1%和35.5%、48.5%、16.0%,兩組分佈差異無統計學意義(χ~2=0.75,P=0.69).攜帶Hap4-GC單體型或Hap4/Hap2單體型對者患肺癌的風險增加,OR值分彆為1.70(95%CI:1.24～2.31)和1.75(95%CI:1.11～2.76),NBS1基因多態與吸煙有聯閤作用(P＜0.05).結論 NBS1 rs1805794 G/G基因型可能是肺癌的易感基因型,rs1805794和rs2735383位點構建的Hap4-GC單體型及Hap4/Hap2單體型對可能是肺癌的易感單體型和單體型對.
목적 탐토DNA쌍련단렬수복기인NBS1다태성여폐암유전역감성적관계.방법 채용병례대조설계,응용PCR-RFLP기술검측575례환자화575명대조적NBS1기인다태.결과 대조조화병례조NBS1 rs1805794적C/C、C/G、G/G기인형빈솔분별위25.9%、51.8%、22.3%화20.5%、52.3%、27.1%,량조분포차이유통계학의의(χ~2=6.38,P=0.04),휴대C/G+G/G기인형개체환폐암적풍험시휴대C/C기인형자적1.46배(OR=1.46,95%C1:1.09～1.97).대조조화병례조NBS1 rs2735383적G/G、G/C、C/C기인형빈솔분별위37.9%、47.0%、15.1%화35.5%、48.5%、16.0%,량조분포차이무통계학의의(χ~2=0.75,P=0.69).휴대Hap4-GC단체형혹Hap4/Hap2단체형대자환폐암적풍험증가,OR치분별위1.70(95%CI:1.24～2.31)화1.75(95%CI:1.11～2.76),NBS1기인다태여흡연유연합작용(P＜0.05).결론 NBS1 rs1805794 G/G기인형가능시폐암적역감기인형,rs1805794화rs2735383위점구건적Hap4-GC단체형급Hap4/Hap2단체형대가능시폐암적역감단체형화단체형대.
Objective To study the association between DNA double-strand break repair gene NBS1(nijmegen breakage syndrome gene)polymorphisms and the susceptibility to lung cancer.Methods A case-control study design was applied.PCR-RFLP was used to identify NBS1 polymorphisms among 575 lung cancer cases and 575 controls.Results The frequencies of C/C,C/G and G/G genotypes at NBS1 rs 1805794 site were 25.9%,51.8%,22.3% among controls compared to 20.5%,52.3%,27.1% among cases.There was significant difference between controls and cases(χ~2=6.38,P=0.04).Individuals carrying C/G + G/G genotypes had an increased risk for lung cancer (OR=1.46,95%CI:1.09-1.97)compared to the C/C genotype.The frequencies of G/G,G/C and C/C genotypes at NBS1 rs2735383 site were 37.9%,47.0%,15.1% among controls compared to 35.5%,48.5%,16.0% among cases,with no significant difference between the two groups(χ~2=0.75,P=0.69).Individuals earning Hap4-GC haplotype(OR=1.70,95%CI:1.24-2.31)and Hap4/Hap2 dihaplotype(OR=1.75,95%CI:1.11-2.76)had an increased risk on lung cancer.Joint associations of smoking and the NBS1 polymorphism with the risk of lung cancer were observed(P＜0.05).Conclusion The G/G genotype at NBS1 rs1805794 site and the Hap4-GC haplotype and Hap4/Hap2 dihaplotype from rs1805794 and rs2735383 were both associated with lung cancer.
