中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
6期
708-711
,共4页
杨光%姚丽华%孙利兵%薛景
楊光%姚麗華%孫利兵%薛景
양광%요려화%손리병%설경
低剂量辐射%CIK细胞%增殖%表型%杀伤活性
低劑量輻射%CIK細胞%增殖%錶型%殺傷活性
저제량복사%CIK세포%증식%표형%살상활성
Low dose irradiation%Cytokine-induced killer cells%Proliferation%Phenotype%Killing activity
目的 观察低剂量辐射对恶性肿瘤患者自体CIK细胞增殖、表型和杀伤活性的影响,为临床应用CIK细胞过继免疫治疗提供依据.方法 取10例恶性肿瘤患者外周血,常规分离出单个核细胞,用不同细胞因子培养诱导CIK细胞.培养10 d后,以30、50、80、100和200 mGy X射线照射,24 h后采用3H-TdR掺入法检测对CIK细胞增殖的影响;流式细胞术检测对CD3+CD56+表型CIK细胞百分率的影响;3H-TdR释放法检测对其杀伤活性的影响.采用3H-TdR释放法检测80 mCyX射线照射对自体CIK细胞在12、24、48和72 h杀伤活性的影响,及80 mGy连续照射3 d对CIK细胞杀伤活性的影响.结果 CIK细胞增殖活性与对照组(0 mGy)相比均有明显增高(t=2.2、3.5、3.3和2.2,P＜0.05).50、80和100 mGy组的CD3+CD56+细胞的百分率均有显著性增高(t=2.3、4.2和2.4,P＜0.05).CIK细胞接受LDI,80 mGy组和100 mGy组与对照组(0 mGy)相比,CIK细胞杀伤活性均有显著性增高(t=3.3和2.3,P＜0.05).80 mGy照射后24 h,CIK细胞的杀伤活性出现高峰,为(54.2±5.0)%(f=3.2,P＜0.01),48和72 h下降到正常水平.80 mGy连续照射3 d,24和48 h CIK细胞杀伤活性分别为(55.2±5.3)%和(61.9±4.4)%,72 h达到最高水平为(67.2±5.7)%(t=2,6、4.7和5.7,P＜0.05).结论 低剂量辐射对肿瘤患者CIK细胞显示兴奋效应,具有临床应用前景.
目的 觀察低劑量輻射對噁性腫瘤患者自體CIK細胞增殖、錶型和殺傷活性的影響,為臨床應用CIK細胞過繼免疫治療提供依據.方法 取10例噁性腫瘤患者外週血,常規分離齣單箇覈細胞,用不同細胞因子培養誘導CIK細胞.培養10 d後,以30、50、80、100和200 mGy X射線照射,24 h後採用3H-TdR摻入法檢測對CIK細胞增殖的影響;流式細胞術檢測對CD3+CD56+錶型CIK細胞百分率的影響;3H-TdR釋放法檢測對其殺傷活性的影響.採用3H-TdR釋放法檢測80 mCyX射線照射對自體CIK細胞在12、24、48和72 h殺傷活性的影響,及80 mGy連續照射3 d對CIK細胞殺傷活性的影響.結果 CIK細胞增殖活性與對照組(0 mGy)相比均有明顯增高(t=2.2、3.5、3.3和2.2,P＜0.05).50、80和100 mGy組的CD3+CD56+細胞的百分率均有顯著性增高(t=2.3、4.2和2.4,P＜0.05).CIK細胞接受LDI,80 mGy組和100 mGy組與對照組(0 mGy)相比,CIK細胞殺傷活性均有顯著性增高(t=3.3和2.3,P＜0.05).80 mGy照射後24 h,CIK細胞的殺傷活性齣現高峰,為(54.2±5.0)%(f=3.2,P＜0.01),48和72 h下降到正常水平.80 mGy連續照射3 d,24和48 h CIK細胞殺傷活性分彆為(55.2±5.3)%和(61.9±4.4)%,72 h達到最高水平為(67.2±5.7)%(t=2,6、4.7和5.7,P＜0.05).結論 低劑量輻射對腫瘤患者CIK細胞顯示興奮效應,具有臨床應用前景.
목적 관찰저제량복사대악성종류환자자체CIK세포증식、표형화살상활성적영향,위림상응용CIK세포과계면역치료제공의거.방법 취10례악성종류환자외주혈,상규분리출단개핵세포,용불동세포인자배양유도CIK세포.배양10 d후,이30、50、80、100화200 mGy X사선조사,24 h후채용3H-TdR참입법검측대CIK세포증식적영향;류식세포술검측대CD3+CD56+표형CIK세포백분솔적영향;3H-TdR석방법검측대기살상활성적영향.채용3H-TdR석방법검측80 mCyX사선조사대자체CIK세포재12、24、48화72 h살상활성적영향,급80 mGy련속조사3 d대CIK세포살상활성적영향.결과 CIK세포증식활성여대조조(0 mGy)상비균유명현증고(t=2.2、3.5、3.3화2.2,P＜0.05).50、80화100 mGy조적CD3+CD56+세포적백분솔균유현저성증고(t=2.3、4.2화2.4,P＜0.05).CIK세포접수LDI,80 mGy조화100 mGy조여대조조(0 mGy)상비,CIK세포살상활성균유현저성증고(t=3.3화2.3,P＜0.05).80 mGy조사후24 h,CIK세포적살상활성출현고봉,위(54.2±5.0)%(f=3.2,P＜0.01),48화72 h하강도정상수평.80 mGy련속조사3 d,24화48 h CIK세포살상활성분별위(55.2±5.3)%화(61.9±4.4)%,72 h체도최고수평위(67.2±5.7)%(t=2,6、4.7화5.7,P＜0.05).결론 저제량복사대종류환자CIK세포현시흥강효응,구유림상응용전경.
Objective To observe the effects of low dose irradiation (LDI) on autoiogous CIK cell proliferation, phenotype and killing activity in tumor patients, and to provide the evidence for clinical application of adoptive immunotherapy with CIK cells. Methods Peripheral blood mononuclear cells (PBMC) were separated from 10 patients with malignant tumor, and CIK cells were cultured with different cytokines. (1) After 10 d culture, C1K cells were irradiated with different doses as 30, 50, 80, 100 and 200 Gy of X-rays was also detected. The CIK cell proliferation and killing activity were measured with 3H-TdR incorporation assay and 3H-TdR release assay, respectively and the percentage variation of CD3 +CD56 + were measured with flow cytometry after 24 h. ( 1 ) Autologous CIK cells were irradiated with 80 mGy X-rays. At different culture time ( 12, 24, 48, 72 h) after irradiation, the killing activity was measured with 3H-TdR release assay. (3) The effect of 3d low dose irradiation of 80 mGy X-rays on thekilling activity of CIK cells was also detected. Results After the CIK cells were irradiated with different doses as 50, 80, 100, 200 mGy of X-rays, the CPM values were 20 048.6 ± 2332. 2 ( t = 2.2, P ＜0.05), 21 832.2 ±2975.9 (t=3.5, P＜0.01), 21 000.3 ±2451.1 (t=3.3, P＜0.01), 19908.1 ±2051.0 ( t = 2.2, P ＜ 0.05 ), respectively and the proliferation of CIK cells were significantly higher than that of control group. The CD3 + CD56 + cell percentage of 50, 80, 100 mGy groups were ( 30.3 ±3.8)% (t=2.3, P＜0.05), (32.3±3.4)% (t=4.2, P＜0.01), (29.742.9)% (t = 2.4, P＜0.05 ), respectively. The killing activity of CIK cells of 80, 100 mGy groups were 55.2 ± 5.0 ( t = 3.3, P ＜ 0.01 ), 52.8 ± 4.1 ( t = 2.3, P ＜ 0.05 ), respectively. The killing activity of CIK cells up-regulated significantly at 24 h, dropped to normal levels at 48 h and 72 h. After 80 mGy X-ray irradiation for 3 consecutive days, the killing activity of CIK cells at different time points were 55.2 ± 5.3 (t = 2.6, P ＜0.05),61.9 ± 4.4 (t = 4.7, P ＜0.01), 67.2 ±5.7 (t = 5.7, P ＜0.01) for 24, 48, 72 h,respectively. Conclusions LDI might have the hormesis effect on CIK cells.
