国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2010年
11期
656-660
,共5页
哮喘%CD4+CD25+T细胞%地塞米松
哮喘%CD4+CD25+T細胞%地塞米鬆
효천%CD4+CD25+T세포%지새미송
Asthma%CD4+CD25+T cells%Dexametasone
目的 研究支气管哮喘(简称哮喘)大鼠模型支气管肺泡灌洗液(BALF)、血液、脾脏CD4+CD25+T细胞的变化,及地塞米松对CD4+CD25+T细胞的影响.方法 50只SD大鼠随机分为5组,空白对照(A)组,哮喘(B)组,地塞米松1(C)组、地塞米松2(D)组,地塞米松3(E)组.A组第l天给予腹腔注射生理盐水l ml,第15～21天每天给予生理盐水雾化.B、C、D、E组用卵蛋白建立哮喘大鼠模型,第1天,每只大鼠腹腔注射抗原l ml(卵蛋白1 mg+灭活百日咳杆菌9×106个+氢氧化铝干粉100 mg)混悬液,第15～21天给予1%的卵蛋白雾化30 min,C、D、E组于雾化后分别给予腹腔注射地塞米松0.2 mg/kg、1 mg/kg、2 mg/kg.采用流式细胞仪检测的方法 ,观察大鼠体内BALF、外周血、脾脏CD4+CD25+T细胞的变化及使用不同剂量地塞米松后对其的影响.结果 B组BALF、外周血、脾脏CD4+CD25+T细胞表达占CD4+T细胞的百分比分别是(42.21±5.62)%、(12.69±2.70)%、(11.15±1.05)%,A组结果 分别是(18.76±5.85)%、(6.21±1.73)%、(7.85±2.13)%.B组与A组比较,差异均具有统计学意义(P＜0.01,P＜0.01,P＜0.05);C组、D组、E组BALF中CD4+CD25+T细胞占CD4+T细胞的百分比表达分别是(10.49±4.03)%、(13.28±5.12)%、(7.51±5.39)%,显著低于A组和B组,(P＜0.05,P＜0.01);外周血中,C组(6.03±1.43)%、D组(4.88±0.95)%与A组(6.21±1.73)%比较,差异无统计学意义,E组(3.49±0.62)%与C组、A组比较,差异有统计学意义(P＜0.05).脾脏中,C组(7.25±1.82)%、D组(8.63±3.18)%与A组(7.85±2.13)%比较,差异无统计学意义,E组(3.38±1.37)%与C组、D组、A组比较,差异有统计学意义(P＜0.05).结论 CD4+CD25+T细胞在哮喘大鼠体内有明显的优势表达,可能是哮喘发病的机制之一.地塞米松可以抑制CD4+CD25+T细胞的表达.BALF内CD4+CD25+T细胞的变化与外周血和脾脏的变化具有一致性,监测外周血或脾脏CD4+CD25+T细胞变化可了解肺部情况.
目的 研究支氣管哮喘(簡稱哮喘)大鼠模型支氣管肺泡灌洗液(BALF)、血液、脾髒CD4+CD25+T細胞的變化,及地塞米鬆對CD4+CD25+T細胞的影響.方法 50隻SD大鼠隨機分為5組,空白對照(A)組,哮喘(B)組,地塞米鬆1(C)組、地塞米鬆2(D)組,地塞米鬆3(E)組.A組第l天給予腹腔註射生理鹽水l ml,第15～21天每天給予生理鹽水霧化.B、C、D、E組用卵蛋白建立哮喘大鼠模型,第1天,每隻大鼠腹腔註射抗原l ml(卵蛋白1 mg+滅活百日咳桿菌9×106箇+氫氧化鋁榦粉100 mg)混懸液,第15～21天給予1%的卵蛋白霧化30 min,C、D、E組于霧化後分彆給予腹腔註射地塞米鬆0.2 mg/kg、1 mg/kg、2 mg/kg.採用流式細胞儀檢測的方法 ,觀察大鼠體內BALF、外週血、脾髒CD4+CD25+T細胞的變化及使用不同劑量地塞米鬆後對其的影響.結果 B組BALF、外週血、脾髒CD4+CD25+T細胞錶達佔CD4+T細胞的百分比分彆是(42.21±5.62)%、(12.69±2.70)%、(11.15±1.05)%,A組結果 分彆是(18.76±5.85)%、(6.21±1.73)%、(7.85±2.13)%.B組與A組比較,差異均具有統計學意義(P＜0.01,P＜0.01,P＜0.05);C組、D組、E組BALF中CD4+CD25+T細胞佔CD4+T細胞的百分比錶達分彆是(10.49±4.03)%、(13.28±5.12)%、(7.51±5.39)%,顯著低于A組和B組,(P＜0.05,P＜0.01);外週血中,C組(6.03±1.43)%、D組(4.88±0.95)%與A組(6.21±1.73)%比較,差異無統計學意義,E組(3.49±0.62)%與C組、A組比較,差異有統計學意義(P＜0.05).脾髒中,C組(7.25±1.82)%、D組(8.63±3.18)%與A組(7.85±2.13)%比較,差異無統計學意義,E組(3.38±1.37)%與C組、D組、A組比較,差異有統計學意義(P＜0.05).結論 CD4+CD25+T細胞在哮喘大鼠體內有明顯的優勢錶達,可能是哮喘髮病的機製之一.地塞米鬆可以抑製CD4+CD25+T細胞的錶達.BALF內CD4+CD25+T細胞的變化與外週血和脾髒的變化具有一緻性,鑑測外週血或脾髒CD4+CD25+T細胞變化可瞭解肺部情況.
목적 연구지기관효천(간칭효천)대서모형지기관폐포관세액(BALF)、혈액、비장CD4+CD25+T세포적변화,급지새미송대CD4+CD25+T세포적영향.방법 50지SD대서수궤분위5조,공백대조(A)조,효천(B)조,지새미송1(C)조、지새미송2(D)조,지새미송3(E)조.A조제l천급여복강주사생리염수l ml,제15～21천매천급여생리염수무화.B、C、D、E조용란단백건립효천대서모형,제1천,매지대서복강주사항원l ml(란단백1 mg+멸활백일해간균9×106개+경양화려간분100 mg)혼현액,제15～21천급여1%적란단백무화30 min,C、D、E조우무화후분별급여복강주사지새미송0.2 mg/kg、1 mg/kg、2 mg/kg.채용류식세포의검측적방법 ,관찰대서체내BALF、외주혈、비장CD4+CD25+T세포적변화급사용불동제량지새미송후대기적영향.결과 B조BALF、외주혈、비장CD4+CD25+T세포표체점CD4+T세포적백분비분별시(42.21±5.62)%、(12.69±2.70)%、(11.15±1.05)%,A조결과 분별시(18.76±5.85)%、(6.21±1.73)%、(7.85±2.13)%.B조여A조비교,차이균구유통계학의의(P＜0.01,P＜0.01,P＜0.05);C조、D조、E조BALF중CD4+CD25+T세포점CD4+T세포적백분비표체분별시(10.49±4.03)%、(13.28±5.12)%、(7.51±5.39)%,현저저우A조화B조,(P＜0.05,P＜0.01);외주혈중,C조(6.03±1.43)%、D조(4.88±0.95)%여A조(6.21±1.73)%비교,차이무통계학의의,E조(3.49±0.62)%여C조、A조비교,차이유통계학의의(P＜0.05).비장중,C조(7.25±1.82)%、D조(8.63±3.18)%여A조(7.85±2.13)%비교,차이무통계학의의,E조(3.38±1.37)%여C조、D조、A조비교,차이유통계학의의(P＜0.05).결론 CD4+CD25+T세포재효천대서체내유명현적우세표체,가능시효천발병적궤제지일.지새미송가이억제CD4+CD25+T세포적표체.BALF내CD4+CD25+T세포적변화여외주혈화비장적변화구유일치성,감측외주혈혹비장CD4+CD25+T세포변화가료해폐부정황.
Objective To investigate the change of CD4+ CD25+ T cells in bronchoalvelar lavage fluid (BALF), blood and spleen of asthmatic rat and the effect of dexamethasone on CD4 + CD25 + T cells.Methods Fifty SD rats were randomly divided into five groups with 10 rats in each group:control group (A), asthmatic group (B), dexamethasone low-dose group (C), dexamethasone mid-dose group (D),dexamethasone high-dose group (E). Group A was established by saline. The rat was injected saline 1 ml in 1 day and challenged from days 15 to 21 by saline 10 ml. The group B,C,D,E,were sensitized on days 1 by injected OVA 1mg and 1 % OVA aerosol challenged from days 15 to 21. The group C, D, E were injected dexamethasone(0. 2 mg/kg, 1 mg/kg,2 mg/kg) after challenges 30 minutes. CD4+ CD25+ T cells in BALF,blood and spleen are measured by flow cytometric analysis. Results BALF, blood and spleen's ratio of CD4+ CD25+ T cells to CD4+ T cells of group B were significantly higher than those in group A [(42.21±5.62)% vs (18.76±5.85)%, P ＜0. 01],[(12. 69±2.70)% vs (6.27±1.73)%, P＜0.01],[(11. 15± 1.05)% vs (7. 85±2.13)%, P ＜0.05]. In BALF, group C(10.49±4.03)%,group D (13.28±5.12)%, group E (7.51 ± 5.3)%, compared with group A (18.76 ±5.85)% group B (42.21± 5.62) %, the differences were significant ( all P ＜ 0. 05 ). In blood, group C ( 6.03 ± 1.43 ) %,group D(4. 88±0.95)%, compared with group A(6.21 ± 1.73)%, the differences were no significant.Group E( 3.49 ± 0.62 ) %, compared with group A ( 6.21 ± 1.73 ) %, group C ( 6.03 ± 1.43 ) %, the differences were significant, (allP ＜0.05). In spleen,group C (7. 25±1.82) % ,group D(8. 63±3.18) %,compared with group A (7.85 ± 2. 13) %, the differences were no significant. Group E ( 3.38 ± 1.37 ) %compared with group A ( 7.85 ± 2.13 ) %, group C ( 7.25 ± 1.82 ) %, group D ( 8. 63 ± 3.18 ) %, the differences were significant, ( P ＜0.05). Conclusions The ratio of CD4+ CD25+ T cells to CD4+ T cells of asthmatic rat exit predominance expression, which may be one of mechanisms of asthma pathogenesis.Dexamethasone can depress CD4+ CD25+ T cells predominance expression. The changes of CD4+ CD25+ T cells in BALF is consistent with that in the blood and spleen,which suggested that monitoring the changes of CD4+ CD25+ T cells in blood and spleen can be useful to understand the situatioan of BALF.
