CAJ | 학술논문

目的探讨CD4+CD25+节性T细胞(CD4+CD25+Treg)在特应性皮炎(AD)发病中的作用机制及临床意义.方法流式细胞仪分析AD患者外周血中CD4+CD25+Treg细胞数量,实时荧光定量PCR检测外周血单核细胞(PBMC)中Foxp3 mRNA水平,ELISA检测血清中IL-2、IL-4、IL-10、IFN-γ水平.结果 AD患者外周血中CD4+CD25+Treg细胞占CD3+T细胞及CD4+T细胞的比例均明显低于正常人对照组(t'=3.775、4.533,P值均＜0.01);外周血中CD4+CD25+Treg细胞占CD3+T细胞比例在AD患者急性期明显低于慢性期(t=2.217,P＜0.05),而在急性期与亚急性期、亚急性期与慢性期之间差异均无统计学意义(t=1.558、0.49,P值均＞0.05).AD患者PBMC中Foxp3 mRNA的水平低于正常人对照组(z=-2.368,P＜0.05);其外周血中CD4+CD25+Treg细胞与血清中IL-2和IL-10成正相关(r=0.512、0.494,P值均＜0.05),与IL-4和IFN-γ的相关性无统计学意义(r=-0.110、-0.237,P值均＞0.05).结论在AD患者中,外周血中CD4+CD25+Treg细胞数量及Foxp3 mRNA水平均下降,从而可能减少对Th2细胞增生及其细胞因子分泌的抑制,使Th2占优势,参与AD的发病.
목적 탐토CD4+CD25+절성T세포(CD4+CD25+Treg)재특응성피염(AD)발병중적작용궤제급림상의의.방법 류식세포의분석AD환자외주혈중CD4+CD25+Treg세포수량,실시형광정량PCR검측외주혈단핵세포(PBMC)중Foxp3 mRNA수평,ELISA검측혈청중IL-2、IL-4、IL-10、IFN-γ수평.결과 AD환자외주혈중CD4+CD25+Treg세포점CD3+T세포급CD4+T세포적비례균명현저우정상인대조조(t'=3.775、4.533,P치균＜0.01);외주혈중CD4+CD25+Treg세포점CD3+T세포비례재AD환자급성기명현저우만성기(t=2.217,P＜0.05),이재급성기여아급성기、아급성기여만성기지간차이균무통계학의의(t=1.558、0.49,P치균＞0.05).AD환자PBMC중Foxp3 mRNA적수평저우정상인대조조(z=-2.368,P＜0.05);기외주혈중CD4+CD25+Treg세포여혈청중IL-2화IL-10성정상관(r=0.512、0.494,P치균＜0.05),여IL-4화IFN-γ적상관성무통계학의의(r=-0.110、-0.237,P치균＞0.05).결론 재AD환자중,외주혈중CD4+CD25+Treg세포수량급Foxp3 mRNA수평균하강,종이가능감소대Th2세포증생급기세포인자분비적억제,사Th2점우세,삼여AD적발병.
Objective To explore the action mechanism and clinical significance of CD4+CD25+ regulatory T (Treg) cells in the development of atopic dermatitis (AD). Methods Peripheral blood mononuclear cells (PBMC) were obtained from 46 patients with AD and 20 normal human controls. Flow cytometry was performed to detect the number of CD4+CD25+ Treg cells, real-time fluorescence PCR assay to measure the Foxp3 mRNA level in PBMC, ELISA to determine the serum levels of IL-2, IL-4, IL-10 and IFN-γ. Results A statistical decrease was observed in the percentages of peripheral CD4+CD25+ Treg cells among CD3+ T cells and CD4+ T cells in AD patients compared with normal controls (t' = 3.775, 4.533, both P＜ 0.01 ), and in the percentage of peripheral CD4+CD25+ Treg cells among CD3+ T cells in patients with acute AD compared with those with chronic AD (t = 2.217, P ＜ 0.05), but no significant difference was noted between patients with acute AD and those with subacute AD or between those with subacute AD and those with chronic AD in the percentage of peripheral CD4+CD25+ Treg cells among CD3+ T cells (t = 1.558, 0.49, both P ＞ 0.05). The mRNA level of Foxp3 in PBMC from AD patients was statistically decreased compred with that from normal controls (z =-2.368, P ＜ 0.05 ). The count of CD4+CD25+ Treg cells was positively correlated with serum levels of IL-2 and IL-10 (r = 0.512, 0.494, both P ＜ 0.05), but had no significant correlation with serum levels of IL-4 and IFN-γ (r = -0.110, -0.237, both P ＞ 0.05). Conclusions In AD patients, there is a decrease in the count of CD4+CD25+ Treg cells and in the level of Foxp3 mRNA, which may suppress the proliferation of and secretion of Foxp3 mRNA by Th2 cells, lead to Th2 predominance, participate in the development of AD.