中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2011年
7期
468-472
,共5页
胡晓%朱加应%黄伟琨%万兴%王建怡
鬍曉%硃加應%黃偉琨%萬興%王建怡
호효%주가응%황위곤%만흥%왕건이
脑脊髓炎,自身免疫性,实验性%雌激素类%基质金属蛋白酶9%小鼠
腦脊髓炎,自身免疫性,實驗性%雌激素類%基質金屬蛋白酶9%小鼠
뇌척수염,자신면역성,실험성%자격소류%기질금속단백매9%소서
Encephalomyelitis,autoimmune,experimental%Estrogens%Matrix metalloproteinase 9%Mice
目的 探讨雌激素对实验性自身免疫性脑脊髓炎(EAE)小鼠中枢神经系统(CNS)中基质金属蛋白酶-9(MMP-9)表达的影响.方法 用MeG35-55多肽诱发60只EAE小鼠模型,做去卵巢术.分为治疗组和对照组,治疗组予雌激素治疗.比较2组EAE小鼠的临床症状评分.取脑和脊髓,行HE染色观察各组EAE小鼠炎症反应;实时荧光定量PCR及免疫荧光染色法检测各组EAE小鼠CNS中MMP-9的表达.结果 治疗组EAE小鼠与对照组相比临床症状减轻(治疗组3.23±0.83,对照组1.62±1.00,t=3.811,P＜0 05)、发病率降低(治疗组8/30,对照组28/30);HE染色显示治疗组炎症细胞浸润(急性期0.895±0.206,缓解期0.752±0.302,慢性期0.732±0.183)较对照组(急性期3.472±0.635,缓解期2.881±0.662,慢性期1.891±0.482)减少(t=8.622、6.543、5.027,均P＜0.01),实时荧光定量PCR及免疫荧光染色结果示治疗组EAE小鼠CNS中MMP-9表达降低.结论 雌激素能抑制EAE小鼠CNS中MMP-9的表达,减轻EAE小鼠临床症状及炎症反应.
目的 探討雌激素對實驗性自身免疫性腦脊髓炎(EAE)小鼠中樞神經繫統(CNS)中基質金屬蛋白酶-9(MMP-9)錶達的影響.方法 用MeG35-55多肽誘髮60隻EAE小鼠模型,做去卵巢術.分為治療組和對照組,治療組予雌激素治療.比較2組EAE小鼠的臨床癥狀評分.取腦和脊髓,行HE染色觀察各組EAE小鼠炎癥反應;實時熒光定量PCR及免疫熒光染色法檢測各組EAE小鼠CNS中MMP-9的錶達.結果 治療組EAE小鼠與對照組相比臨床癥狀減輕(治療組3.23±0.83,對照組1.62±1.00,t=3.811,P＜0 05)、髮病率降低(治療組8/30,對照組28/30);HE染色顯示治療組炎癥細胞浸潤(急性期0.895±0.206,緩解期0.752±0.302,慢性期0.732±0.183)較對照組(急性期3.472±0.635,緩解期2.881±0.662,慢性期1.891±0.482)減少(t=8.622、6.543、5.027,均P＜0.01),實時熒光定量PCR及免疫熒光染色結果示治療組EAE小鼠CNS中MMP-9錶達降低.結論 雌激素能抑製EAE小鼠CNS中MMP-9的錶達,減輕EAE小鼠臨床癥狀及炎癥反應.
목적 탐토자격소대실험성자신면역성뇌척수염(EAE)소서중추신경계통(CNS)중기질금속단백매-9(MMP-9)표체적영향.방법 용MeG35-55다태유발60지EAE소서모형,주거란소술.분위치료조화대조조,치료조여자격소치료.비교2조EAE소서적림상증상평분.취뇌화척수,행HE염색관찰각조EAE소서염증반응;실시형광정량PCR급면역형광염색법검측각조EAE소서CNS중MMP-9적표체.결과 치료조EAE소서여대조조상비림상증상감경(치료조3.23±0.83,대조조1.62±1.00,t=3.811,P＜0 05)、발병솔강저(치료조8/30,대조조28/30);HE염색현시치료조염증세포침윤(급성기0.895±0.206,완해기0.752±0.302,만성기0.732±0.183)교대조조(급성기3.472±0.635,완해기2.881±0.662,만성기1.891±0.482)감소(t=8.622、6.543、5.027,균P＜0.01),실시형광정량PCR급면역형광염색결과시치료조EAE소서CNS중MMP-9표체강저.결론 자격소능억제EAE소서CNS중MMP-9적표체,감경EAE소서림상증상급염증반응.
Objective To study the regulation effect of estrogen in expression of matrix metalloproteinase-9 (MMP-9) in the central nervous system (CNS) in mice with experimental autoimmune encephalomyelitis ( EAE).Methods The 60 mice were overiectomized and 2 weeks later EAE was induced with MOG35-55 peptide in these mice.They were divided into a treatment group and a control group.The treatment group was treated with estrogen and the control group was given PBS.Clinical symptoms in these two groups were scored and compared.HE staining was used to observe inflammation in the brain and spinal cord.The MMP-9 expression in the CNS was examined by quantitative real-time PCR and immunofluorescence staining.Results The incidence of disease was lower (treatment and control group were 8/30 and 28/30 respectively) and clinical symptoms were milder (treatment and control group were 3.23±0.83 and 1.62 ±1.00 respectively,t=3.811 and P＜0.05) in the treatment group than those in the control group.HE staining showed the decreased infiltration of inflammatory cell in the treatment group (Treatment group:inflammatory score were 0.895 ±0.206,0.752 ±0.302,0.732 ±0.183 in acute,relief and chronic phase respectively;Control group:inflammatory score were 3.472 ±0.635,2.881 ±0.662,1.891 ± 0.482 in acute,relief and chronic phase respectively.t = 8.622,6.543 and 5.027,all P ＜ 0.05).The quantitative real-time PCR and immunofluorescence staining showed that the expression of MMP9 in the CNS was decreased in the treatment group.Conclusion Estrogen may decrease MMP-9 expression in the CNS,reduce inflammation and clinical symptoms in mice with EAE.
