CAJ | 학술논문

目的应用超声造影定量探讨3-(5’-羟甲基-2’-呋喃基)-1-甲苯(YC-1)治疗人胃癌裸鼠皮下移植瘤疗效的价值.方法建立人胃癌SGC7901/DDP细胞株裸鼠皮下移植瘤模型,将24只裸鼠随机分为4组:对照组(给予二甲基亚砜)、DDP组(给予顺铂)、YC-1组(给予YC-1)和YC-1+DDP组(给予YC-1+顺铂).停药1周后行超声造影检查,应用SonoLiver进行定量分析.每一个肿瘤画2个感兴趣区:ROItotal包含整个肿瘤,ROIsmall包含肿瘤中造影增强最强的部分.所得定量参数包括达峰时间(TTP)、上升时间(RT)、渡越时间(mTT)、达峰强度(Imax)、下降支斜率(R10、R20、R30)及上升支斜率(Vasc).结果 ROIsmall及ROItotal上,各超声造影定量参数在对照组与DDP之间以及在YC-1组与YC-1+DDP组之间差异均无统计学意义(P＞0.05).将裸鼠按照是否接受YC-1治疗重新分为YC-1治疗组及非YC-1治疗组.ROItotal上,各超声造影定量参数在YC-1治疗组及非YC-1治疗组间差异均无统计学意义(P＞0.05).ROIsmall上,与非YC-1治疗组相比,YC-1治疗组肿瘤达峰提前(TTP:5.47 ±0.93比8.05±2.00；RT:4.99±0.96比7.67±1.78,P＜0.05),造影剂消退快(mTT:37.49±19.51比98.94±60.97; R10:0.39±0.15比0.55±0.08；R20:0.30 ±0.10比0.43±0.10;R30:0.25±0.08比0.33±0.08,P＜0.05).结论超声造影定量分析可有效评价YC-1对人胃癌裸鼠皮下移植瘤的治疗疗效,ROIsmall较ROItotal更适合用来评价肿瘤抗血管生成治疗的疗效.
목적 응용초성조영정량탐토3-(5’-간갑기-2’-부남기)-1-갑분(YC-1)치료인위암라서피하이식류료효적개치.방법 건립인위암SGC7901/DDP세포주라서피하이식류모형,장24지라서수궤분위4조:대조조(급여이갑기아풍)、DDP조(급여순박)、YC-1조(급여YC-1)화YC-1+DDP조(급여YC-1+순박).정약1주후행초성조영검사,응용SonoLiver진행정량분석.매일개종류화2개감흥취구:ROItotal포함정개종류,ROIsmall포함종류중조영증강최강적부분.소득정량삼수포괄체봉시간(TTP)、상승시간(RT)、도월시간(mTT)、체봉강도(Imax)、하강지사솔(R10、R20、R30)급상승지사솔(Vasc).결과 ROIsmall급ROItotal상,각초성조영정량삼수재대조조여DDP지간이급재YC-1조여YC-1+DDP조지간차이균무통계학의의(P＞0.05).장라서안조시부접수YC-1치료중신분위YC-1치료조급비YC-1치료조.ROItotal상,각초성조영정량삼수재YC-1치료조급비YC-1치료조간차이균무통계학의의(P＞0.05).ROIsmall상,여비YC-1치료조상비,YC-1치료조종류체봉제전(TTP:5.47 ±0.93비8.05±2.00；RT:4.99±0.96비7.67±1.78,P＜0.05),조영제소퇴쾌(mTT:37.49±19.51비98.94±60.97; R10:0.39±0.15비0.55±0.08；R20:0.30 ±0.10비0.43±0.10;R30:0.25±0.08비0.33±0.08,P＜0.05).결론 초성조영정량분석가유효평개YC-1대인위암라서피하이식류적치료료효,ROIsmall교ROItotal경괄합용래평개종류항혈관생성치료적료효.
Objective To explore the value of contrast-enhanced ultrasound quantitative analysis in the evaluation of YC-1 effect on transplanted tumor of human gastric carcinoma in nude mice.Methods The transplanted tumor model of human gastric carcinoma in nude mice was established by injecting SGC7901/displatin (DDP) cells into the subcutaneous space of 24 nude mice.The mice were randomized into four groups as control group ( DMSO given),DDP group ( DDP given),YC-1 group (YC-1 given) and YC-1 + DDP group ( both YC-1 and DDP given).Contrast-enhanced ultrasound was performed one week after withdrawl and the dynamic image was analyzed quantitatively using SonoLiver software.For each tumor,two ROIs as ROItotal (including the total tumor) and ROIsmall (including the highest enhanced part of the tumor)were drawn and parameters including time-to-peak (TTP),rise time (RT),mean transit time (mTT),maximun intensity ( Imax),R10,R20 and R30 ( descending velocity-the rate of intensity at TTP + 10/20/30 s to Imax) and ascending velocity (Vasc) were recorded for the statistical analysis.Results No parameter had significant difference between control group and DDP group or between YC-1 group and YC-1 + DDP group in both ROIsamll and ROItotal (P ＞ 0.05 ).The mice were regrouped into YC-1-treated-group and non-YC-1-treated-group.For ROItotal,no parameter had significant difference between the two groups (P ＞ 0.05 ).For ROIsmall,microbubbles in YC-1-treated-group reached the peak earlier (TTP:5.47 ±0.93 vs 8.05 ±2.00; RT:4.99 ±0.96 vs 7.67 ± 1.78,P＜0.05) and regressed faster (mTT:37.49 ± 19.51 vs 98.94 ±60.97; R10:0.39 ±0.15 vs 0.55 ±0.08; R20:0.30 ±0.10 vs 0.43 ±0.10; R30:0.25 ±0.08 vs0.33±0.08) than in non-YC-1-treated-group (P＜0.05).Conclusion Contrast-enhanced ultrasound quantitative analysis could reflect the change of tumor blood supply after antiangiogenesis treatment.ROIsmall was more suitable and sensitive than ROItotal to evaluate antiangiogenesis effect.