CAJ | 학술논문

目的观察低表达蛋白HAX1对Hela细胞维持线粒体膜电位及细胞凋亡的影响.方法利用RNA干扰技术抑制Hela蛋白HAX1表达3 d后,利用阳离子脂质荧光染料JC-I标记法和流式细胞仪检测并比较实验组(蛋白HAX1低表达组)及对照组细胞线粒体膜电位变化趋势.加入H2O2(2 mmol/L)诱导细胞凋亡3 h后利用Annexin V-FITC法检测并比较两组细胞凋亡率.结果对照组细胞线粒体膜电位下降百分比均值为9.52%,实验组细胞线粒体膜电位下降百分比均值为21.12%,实验组细胞线粒体膜电位降低比率多于对照组(P＜0.05).对照组H2O2(2 mmol/L)诱导细胞凋亡率均值为21.80%,实验组H2O2(2 mmol/L)诱导细胞凋亡率均值为31.73%.实验组细胞凋亡率高于对照组(P＜0.05).结论低表达蛋白HAX1不仅可以降低Hela细胞线粒体膜电位稳定性,而且促进Hela凋亡.
목적 관찰저표체단백HAX1대Hela세포유지선립체막전위급세포조망적영향.방법 이용RNA간우기술억제Hela단백HAX1표체3 d후,이용양리자지질형광염료JC-I표기법화류식세포의검측병비교실험조(단백HAX1저표체조)급대조조세포선립체막전위변화추세.가입H2O2(2 mmol/L)유도세포조망3 h후이용Annexin V-FITC법검측병비교량조세포조망솔.결과 대조조세포선립체막전위하강백분비균치위9.52%,실험조세포선립체막전위하강백분비균치위21.12%,실험조세포선립체막전위강저비솔다우대조조(P＜0.05).대조조H2O2(2 mmol/L)유도세포조망솔균치위21.80%,실험조H2O2(2 mmol/L)유도세포조망솔균치위31.73%.실험조세포조망솔고우대조조(P＜0.05).결론 저표체단백HAX1불부가이강저Hela세포선립체막전위은정성,이차촉진Hela조망.
Objective To investigate the effect of the down-regulation of HAX1 protein on maintenance of mitochondria membrane potential and apoptosis of Hela cells.Methods After 3 days of inhibiting the expression of HAX1 protein by using RNAi technology,the changes in mitoehondria membrane potential of Hela cells were observed and compared between experimental group and control group by JC-I labeling method and flow cytometry.Three h after addition of H2O2(2 retool/L)to induce apoptosis,the apoptosis rate was detected by using Annexin V-FITC technique.Results The decreasing percent of cellular mitochondria membrane potential in control and experimental groups was 9.52%and 21.12%in average respectively(P＜0.05).The H2O2(2 mmol/L)-induced apoptosis rate in control and experimental groups was 21.80%and 31.73%in average respectively(P＜0.05).Conclusion Down-regulation of HAX1 protein can not only reduce the stability of mitochondria membrane potential,but also induce apoptosis of Hela ceils.