CAJ | 학술논문

目的研究基质金属蛋白酶-2(MMP-2)及组织金属蛋白酶抑制因子-3(TIMP-3)在贲门癌中的表达及与病理分期间的关系.方法 115例贲门癌患者手术切除标本,以其中40例患者癌旁非肿瘤胃组织(离癌灶边缘>5 cm)为正常对照,二步法免疫组织化学检测MMP-2、TIMP-3的表达,分析二者表达与病理分期之间的关系;并通过Kaplan-Meier寿命表法分析与预后的关系;COX模型多因素分析贲门癌的独立预后因子.结果 MMP-2在贲门癌和癌旁对照组织阳性表达率分别为57.4%(66例)和40.0%(16例)(P<0.05),其表达与肿瘤大小、肿瘤浸润深度、淋巴结转移及病期正相关,与贲门癌患者预后呈负相关(P<0.05).TIMP-3在贲门癌和癌旁对照组织阳性表达率分别为48.7%(56例)和95.0%(38例)(P<0.05),其在贲门癌中的表达与肿瘤大小、肿瘤浸润深度、淋巴结转移及病期负相关,与患者预后正相关.结论 MMP-2表达增加及TIMP-3表达缺失,在贲门癌侵袭、转移中发挥重要作用.联合检测MMP-2与TIMP-3,有助于了解贲门癌的发展和评估贲门癌患者的预后.
목적 연구기질금속단백매-2(MMP-2)급조직금속단백매억제인자-3(TIMP-3)재분문암중적표체급여병리분기간적관계.방법 115례분문암환자수술절제표본,이기중40례환자암방비종류위조직(리암조변연>5 cm)위정상대조,이보법면역조직화학검측MMP-2、TIMP-3적표체,분석이자표체여병리분기지간적관계;병통과Kaplan-Meier수명표법분석여예후적관계;COX모형다인소분석분문암적독립예후인자.결과 MMP-2재분문암화암방대조조직양성표체솔분별위57.4%(66례)화40.0%(16례)(P<0.05),기표체여종류대소、종류침윤심도、림파결전이급병기정상관,여분문암환자예후정부상관(P<0.05).TIMP-3재분문암화암방대조조직양성표체솔분별위48.7%(56례)화95.0%(38례)(P<0.05),기재분문암중적표체여종류대소、종류침윤심도、림파결전이급병기부상관,여환자예후정상관.결론 MMP-2표체증가급TIMP-3표체결실,재분문암침습、전이중발휘중요작용.연합검측MMP-2여TIMP-3,유조우료해분문암적발전화평고분문암환자적예후.
Objective The aim of this study was to explore MMP-2 and TIMP-3 expression in the gastric cardia carcinoma and with the relationship between the pathological stages.Methods Gastric cardia carcinoma surgical resection speciments of 115 cases were for the trial group.paraneoplastic gastric noncancerous tissues of 40 cases (the distance to cancer border>5 cm) as normal controls.The expression of MMP-2 and TIMP-3 was detected by two-step immunohistochemical method.The relationship of MMP-2 and TIMP-3 between clinicopathological stages was analyze. Results MMP-2 in carcinoma of the gastric cardia and the paraneoplastic tissues of organizations positive expression rates were 57.4%(66/115) and 40.0%(16/40) (P<0.05).MMP-2 expression was positively correlated with tumor size,tumor invasion depth,lymphatic metastasis and stage of tumor, which was negatively correlated with the prognosis of gastric cardia carcinoma (P<0.05).TIMP-3 in carcinoma of the gastric cardia and the paraneoplastic tissues of organizations positive expression rates were 48.7%(56/115) and 95.0%(38/40) (P<0.05).TIMP-3 expression was negatively correlated with tumor size,tumor invasion depth,lymphatic metastasis and stage of tumor, which was positively correlated with the prognosis of gastric cardia carcinoma. Conclusion The over expression of MMP-2 and the lost of TIMP-3 may play an important role in the invasion and metastasis of gastric cardia carcinoma.Combined MMP-2 and TIMP-3 detection may help to judge gastric cardia carcinoma development and to assess the prognosis of patients.