CAJ | 학술논문

目的探讨沙利度胺治疗血管发育不良所致消化道出血的机制.方法体外培养人脐静脉内皮细胞至对数生长期,分为空白对照组、溶剂对照组(二甲基亚砜)和不同浓度(10、20、40、60、80、100μg/ml)沙利度胺组,根据加或不加成纤维细胞生长因子(bFGF,10 ng/ml),共分为16组.刺激72 h后,MTT法检测细胞增殖情况,酶联免疫吸附法和实时定量PCR法测定血管内皮生长因子(VEGF)、肿瘤坏死因子-α(TNF-α)表达.结果加或不加bFGF刺激,中、高浓度(≥40/μg/ml)沙利度胺均能抑制人脐静脉内皮细胞增殖.未加bFGF刺激时.20μg/ml沙利度胺能明显抑制VEGF表达.加bFGF刺激时,10 μg/ml沙利度胺即能明显抑制VEGF表达.未检出TNF-α表达.结论体外实验中,沙利度胺能抑制人脐静脉内皮细胞增殖和VEGF表达,从而抑制血管生成,达到治疗血管发育不良所致消化道出血的目的 .
목적 탐토사리도알치료혈관발육불량소치소화도출혈적궤제.방법 체외배양인제정맥내피세포지대수생장기,분위공백대조조、용제대조조(이갑기아풍)화불동농도(10、20、40、60、80、100μg/ml)사리도알조,근거가혹불가성섬유세포생장인자(bFGF,10 ng/ml),공분위16조.자격72 h후,MTT법검측세포증식정황,매련면역흡부법화실시정량PCR법측정혈관내피생장인자(VEGF)、종류배사인자-α(TNF-α)표체.결과 가혹불가bFGF자격,중、고농도(≥40/μg/ml)사리도알균능억제인제정맥내피세포증식.미가bFGF자격시.20μg/ml사리도알능명현억제VEGF표체.가bFGF자격시,10 μg/ml사리도알즉능명현억제VEGF표체.미검출TNF-α표체.결론 체외실험중,사리도알능억제인제정맥내피세포증식화VEGF표체,종이억제혈관생성,체도치료혈관발육불량소치소화도출혈적목적 .
Objective To investigate the mechanism and effect of thalidomide on gastrointestinal bleeding of angiodysplasia. Methods The endothelial cells of human umbilical vein were cultured in vitro to exponential phase of growth, then were divided into blank control, solvent control and different concentrations (10- 100 μg/ml) of thalidomide incubated with or without basic fibroblast growth factor (bFGF). The cell proliferation was measured by MTT assay 72 h after stimulation. The expressions of vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNF-α) were detected by ELISA and real-time PCR, respectively. Results The proliferation of endothelial cells of human umbilical vein was inhibited by thalidomide (≥40 μg/ml) both in presence or absence of bFGF. The expression of VEGF could be inhibited by 20 μg/ml of thalidomide in the absence of bFGF and 10 μg/ml in the presence of hFGF. No expression of TNF-α was detected. Conclusions The in vitro study reveals that thalidomide can inhibit the proliferation and the expression of VEGF, which may treat gastrointestinal bleeding of angiodysplasia by suppressing the angiogenesis.