CAJ | 학술논문

目的检测FAT10与突变型p53在胃癌组织中的表达情况及相互间关系.方法采用免疫组化和逆转录-聚合酶链反应(RT-PCR)方法检测FAT10和突变型p53在62例胃癌患者的胃癌组织、癌旁组织(距癌边缘2～5 cm)及正常胃组织(距癌边缘>5 cm)中的表达水平,统计分析两者表达间的关联性及FAT10与胃癌预后之间的关系.结果 FAT10蛋白和突变型p53蛋白在胃癌组织中的表达阳性率分别为51.61%(32/62)和45.16%(28/62),显著高于癌旁组织[12.90%(8/62)和14.51%(9/62),χ2值分别=21.26和20.69,P值均<0.01]及正常胃组织[6.45%(4/62)和9.68%(6/62),χ2值分别=13.91和19.61,P值均<0.01].胃癌FAT10蛋白及mRNA的表达上升主要与胃癌的淋巴转移及TNM分期密切相关(P值均<0.05).胃癌组织中FAT10表达在蛋白及基因水平均与突变型p53表达呈正相关性(r值分别=0.865和0.761,P值分别<0.05和0.01).FAT10蛋白阳性表达或mRNA表达水平高者,其累计生存时间显著低于FAT10阴性者(P值均<0.05).结论 FAT10与突变型p53在胃癌组织中表达上调且呈正相关性,两者可能共同参与胃癌的形成与发展,FAT10对评价胃癌的预后可能有较好的价值.
목적 검측FAT10여돌변형p53재위암조직중적표체정황급상호간관계.방법 채용면역조화화역전록-취합매련반응(RT-PCR)방법검측FAT10화돌변형p53재62례위암환자적위암조직、암방조직(거암변연2～5 cm)급정상위조직(거암변연>5 cm)중적표체수평,통계분석량자표체간적관련성급FAT10여위암예후지간적관계.결과 FAT10단백화돌변형p53단백재위암조직중적표체양성솔분별위51.61%(32/62)화45.16%(28/62),현저고우암방조직[12.90%(8/62)화14.51%(9/62),χ2치분별=21.26화20.69,P치균<0.01]급정상위조직[6.45%(4/62)화9.68%(6/62),χ2치분별=13.91화19.61,P치균<0.01].위암FAT10단백급mRNA적표체상승주요여위암적림파전이급TNM분기밀절상관(P치균<0.05).위암조직중FAT10표체재단백급기인수평균여돌변형p53표체정정상관성(r치분별=0.865화0.761,P치분별<0.05화0.01).FAT10단백양성표체혹mRNA표체수평고자,기루계생존시간현저저우FAT10음성자(P치균<0.05).결론 FAT10여돌변형p53재위암조직중표체상조차정정상관성,량자가능공동삼여위암적형성여발전,FAT10대평개위암적예후가능유교호적개치.
Objective To investigate the expressions of FAT10 and p53 mutant in gastric cancer tissues and their relations. Methods Immunohistochemistry and RT-PCR were used to detect the expressions of FAT10 and p53 in gastric cancer tissues (n=62), para-cancerous tissues (2-5 cm apart from cancer, n=62), and normal gastric tissues (7>5 cm apart from cancer, n=62). The association of FAT10 with p53 and clinical outcomes were analyzed by Spearman and Pearson correlation. Results The immunohistochemistry examination showed that expressions of FAT10 [51.61%(32/62)] and p53 [45.16% (28/62)] were significantly higher in cancerous tissues than in para-cancerous tissues [12.90%(8/62) and 14.51% (9/62), χ2=21.26 and 20.69, P<0.01] and normal tissues [6.45% (4/62) and 9.68% (6/62), χ2=13.91 and 19.61, P<0.01]. Overexpressions of FAT10 protein and mRNA in cancerous tissues were closely related to lymph node metastasis and TNM staging (both P value<0.05). There was a positive correlation between FAT10 and p53 in protein and mRNA expressions (protein r=0. 865, P<0.05; mRNA r=0.761, P< 0.01). Those with positive expression of FAT10 had lower survival rate compared to those with negative expression (P<0.05). Conclusions The positive relation between over-expression of FAT10 and p53 implicates that both are involved in the gastric carcinogenesis, and FAT10 is a novel gastric cancer marker with prognostic significance.