CAJ | 학술논문

目的探讨乳腺癌患者治疗过程中血浆样本中多药耐药基因(mdr-1)相关的磷-精蛋白(P-gp)的变化,为临床治疗提供参考依据.方法采用生化反应方法.将血浆样本与化学试剂混匀,根据反应前后透光率的不同,计算每个样本P-gp活性的改变以及对长春新碱的相对活性,相对活性大于30%即判定为P-gp阳性.结果治疗前P-gp阳性率为16.2%.化疗3个周期后P-gp阳性率为43.2%,二者差异有统计学意义(P<0.05).治疗前和化疗后P-gp阳性有淋巴结转移的分别为5/6和9/16,无淋巴结转移的分别为1/6和7/16.结论乳腺癌患者血浆P-gp活性测定,对乳腺癌化疗用药选择及其预后判断具有参考价值.
목적 탐토유선암환자치료과정중혈장양본중다약내약기인(mdr-1)상관적린-정단백(P-gp)적변화,위림상치료제공삼고의거.방법 채용생화반응방법.장혈장양본여화학시제혼균,근거반응전후투광솔적불동,계산매개양본P-gp활성적개변이급대장춘신감적상대활성,상대활성대우30%즉판정위P-gp양성.결과 치료전P-gp양성솔위16.2%.화료3개주기후P-gp양성솔위43.2%,이자차이유통계학의의(P<0.05).치료전화화료후P-gp양성유림파결전이적분별위5/6화9/16,무림파결전이적분별위1/6화7/16.결론 유선암환자혈장P-gp활성측정,대유선암화료용약선택급기예후판단구유삼고개치.
Objective To investigate the relative activity of multidrug resistance P-glycoprotein in the plasma of breast cancer patients, analyze the expression of P-glycoprotein before and after chemotherapy and evaluate its clinical value. Methods The plasma of patients and chemical reagents were mixed and distributed into the 96-well plate. The plate was read at 340 nm absorbaace before and after reaction. The variation of absorbance for each well was calculated, and then relative activity of each sample was compared to the referent compound activity. The relative activity of over 30 % was taken as positive expression of the P-gp for each sample. Results The frequency of P-glycoprotein expression was significantly increased from 16.2 % before chemotherapy to 43.2 % after chemotherapy (P<0.05). The positive expression of P-glycoprotein before and after chemotherapy were 5/6 and 9/16, respectively, in patients with positive lymph node metastasis, and 1/6 and 7/16, respectively, in patients with negative lymph node metastasis. Conclusion The results of this preliminary study suggest in P-glycoprotein expression in plasma of patients play an important role in the selection of drugs, which may be useful to evaluate the prognosis for treatment of breast cancer patients.