CAJ | 학술논문

目的　建立表达可溶性HLA-G1蛋白的真核表达载体pcDNA3-sHLA-G1。方法　从绒癌细胞系Jeg-3细胞中提取总RNA，借助RT-PCR技术扩增可溶性HLA-G1的cDNA并把其插入真核表达载体pcDNA3，然后经酶切和测序法鉴定。结果　经酶切鉴定及测序分析，证实已成功构建pcDNA3-sHLA-G1。结论　本研究成功构建可溶性HLA-G1的真核表达载体。
목적　건립표체가용성HLA-G1단백적진핵표체재체pcDNA3-sHLA-G1。방법　종융암세포계Jeg-3세포중제취총RNA，차조RT-PCR기술확증가용성HLA-G1적cDNA병파기삽입진핵표체재체pcDNA3，연후경매절화측서법감정。결과　경매절감정급측서분석，증실이성공구건pcDNA3-sHLA-G1。결론　본연구성공구건가용성HLA-G1적진핵표체재체。
Objective　To construct a recombinant plasmid pcDNA3-sHLA-G1 expressing soluble HLA-G1. Methods　 Total cell RNA was extracted from the cell line Jeg-3 and the cDNA was amplified by RT-PCR； The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results　 After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3-sHLA-G1 had been constructed successfully. Conclusion　 In this study, the recombinant plasmid pcDNA3-sHLA-G1 had been constructed successfully.