CAJ | 학술논문

细胞周期蛋白(cyclin)B1作为调节细胞进行有丝分裂的正性调节因子,在细胞增殖中起到重要作用.本研究旨在评价cyclin B1在急性白血病(AL)发生、发展中的作用及其对AL患者预后判断的价值.采用流式细胞术对136例初治AL成人患者、10例持续完全缓解(CCR)患者及17例正常人进行cyclin B1、p21cipl蛋白表达水平检测和细胞周期分布分析;采用RT-PCR测定Cyclin B1、p21 cipl和增殖细胞核抗原(PCNA)mRNA水平.结果显示:136例初治AL患者cyclin B1蛋白表达水平明显高于正常对照组;复发AL患者的cyclin B1表达水平明显高于正常对照组,但低于初治AL患者;缓解AL患者及CCR的AL患者cyclin B1蛋白表达水平与正常对照比较均无显著性差异;所有cyclin B1高表达的AL患者出现非顺序表达(cyclin B1在G1期出现,有些甚至G2期高表达),cyclin B1与p21cipl的蛋白表达呈负相关;cyclin B1蛋白表达与其mRNA水平呈正相关;cyclin B1表达与PCNA及细胞增殖指数(PI)表达水平呈正相关;cyclin B1表达水平高的AL患者有较高的缓解率和生存率,但有较高的复发率.结论:本研究首次发现cyclin B1在成人AL患者有过度表达和异常分布,认为cyclin B1与AL发生、发展有关,是影响AL患者预后的因素之一.
세포주기단백(cyclin)B1작위조절세포진행유사분렬적정성조절인자,재세포증식중기도중요작용.본연구지재평개cyclin B1재급성백혈병(AL)발생、발전중적작용급기대AL환자예후판단적개치.채용류식세포술대136례초치AL성인환자、10례지속완전완해(CCR)환자급17례정상인진행cyclin B1、p21cipl단백표체수평검측화세포주기분포분석;채용RT-PCR측정Cyclin B1、p21 cipl화증식세포핵항원(PCNA)mRNA수평.결과현시:136례초치AL환자cyclin B1단백표체수평명현고우정상대조조;복발AL환자적cyclin B1표체수평명현고우정상대조조,단저우초치AL환자;완해AL환자급CCR적AL환자cyclin B1단백표체수평여정상대조비교균무현저성차이;소유cyclin B1고표체적AL환자출현비순서표체(cyclin B1재G1기출현,유사심지G2기고표체),cyclin B1여p21cipl적단백표체정부상관;cyclin B1단백표체여기mRNA수평정정상관;cyclin B1표체여PCNA급세포증식지수(PI)표체수평정정상관;cyclin B1표체수평고적AL환자유교고적완해솔화생존솔,단유교고적복발솔.결론:본연구수차발현cyclin B1재성인AL환자유과도표체화이상분포,인위cyclin B1여AL발생、발전유관,시영향AL환자예후적인소지일.
Cyclin B1, a positive regulator, controls mitosis occurrence, plays an important role in cell proliferation.To investigate the clinical significance of cyclin B1, the expression of cyclin B1 in acute leukemia (AL) patients was measured; the expression of cyclin B1 and p21 cipl, and their cell cycle distribution were assayed by flow cytometry in 136 adult patients with newly diagnosed AL, 10 continuous complete remission (CCR) AL and 17 normal controls; the mRNA of cyclin B 1 and p21 cipl, and the proliferation cell nuclear antigen (PCNA) in patients and normal controls were detected with semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The results showed that the expression of cyclin B1 in newly diagonsed AL patients was significantly higher than that in normal controls. For the relapsed AL patients, the cyclin B1 expression was also higher than that in normal controls, but lower than that in newly diagnosed cases, there was no significant difference between the remission cases and normal controls, nor difference between CCR AL patients and normal controls. All patients with high cyclin B1 expression had an unscheduled expression manner, that cyclin B1 protein appeared in G1 phase, and in some case it even higher than that of G2 phase.The response rate (partial remission + complete remission) and survival rate in the cyclin B1 high expressed patients were higher than that of cyclin B1 low expressed patients. The relapse rate in cyclin B1 high expressed patients was higher than that in cyclin B1 normally expressed patients. The survival rate in cyclin B1 high expressed patients was higher than that in cyclin B1 low expression patients. A negative correlation between the expression of cyclin B1 and p21 cipl was observed. Additionally, cyclin B1 protein expression was generally correlated with proliferation index (PI)and proliferation cell nuclear antigen (PCNA). It is concluded that this study demonstrates for the first time cyclin B1 overexpression and abnormally distribution in cell cyclin of newly diagnosed AL patients. It was considered that cyclin B1 may play an important role in leukemic pathogeneses and can be one of the factors influencing the prognosis of AL patients.