家禽科学
傢禽科學
가금과학
POULTRY SCIENCE
2009年
7期
3-6
,共4页
谭淑樱%王海英%祁克宗%彭开松
譚淑櫻%王海英%祁剋宗%彭開鬆
담숙앵%왕해영%기극종%팽개송
鸡%肠黏膜%抗微生物肽%温度%pH值
鷄%腸黏膜%抗微生物肽%溫度%pH值
계%장점막%항미생물태%온도%pH치
chicken%intestinal mucous membrane%antimicrobial peptide%temperature%pH value
取新鲜鸡肠黏膜超声波破碎,乙酸浸提,经Sephadex G-50层析后,收集具有抑菌活性的组分进行超滤离心,抑菌实验表明滤过物具有抑菌活性.滤过物经SDS-PAGE分析为一个条带,分子量约为6.078KD,得到电泳纯的鸡肠道抗微生物肽.该肽具有一定的耐热性,但经90℃以上热处理后活性下降较大;pH值对其活性影响较大,在pH6～8时活性最强.
取新鮮鷄腸黏膜超聲波破碎,乙痠浸提,經Sephadex G-50層析後,收集具有抑菌活性的組分進行超濾離心,抑菌實驗錶明濾過物具有抑菌活性.濾過物經SDS-PAGE分析為一箇條帶,分子量約為6.078KD,得到電泳純的鷄腸道抗微生物肽.該肽具有一定的耐熱性,但經90℃以上熱處理後活性下降較大;pH值對其活性影響較大,在pH6～8時活性最彊.
취신선계장점막초성파파쇄,을산침제,경Sephadex G-50층석후,수집구유억균활성적조분진행초려리심,억균실험표명려과물구유억균활성.려과물경SDS-PAGE분석위일개조대,분자량약위6.078KD,득도전영순적계장도항미생물태.해태구유일정적내열성,단경90℃이상열처리후활성하강교대;pH치대기활성영향교대,재pH6～8시활성최강.
After the intestinal mucous membranes of chicken were treated by ultrasonic treatment and acid extraction,an antimierobial peptide was isolated from the intestine of chicken by Sephadex G-50 gel filtration chromatography and centrifugal ultrafiltration.The filtration material was analyzed by SDS-PAGE and the result showed that an antimierobial peptide was purified and its molecule weight was about 6.078kD.The antimicrobial peptide was heat-resistant,but its activity descended obviously after heated at 90℃.The activity of the antimierobial peptide was affected by pH and it was the strongest at pH6～8.
