海洋通报
海洋通報
해양통보
MARINE SCIENCE BULLETIN
2010年
1期
52-58
,共7页
烟台海域%嗜盐菌%16S rDNA%生物多样性%系统进化分析
煙檯海域%嗜鹽菌%16S rDNA%生物多樣性%繫統進化分析
연태해역%기염균%16S rDNA%생물다양성%계통진화분석
Yantai sea area%halophilic bacterium%16 S rDNA%biodiversity%phylogenetic analysis
为研究山东烟台海域中度嗜盐菌的分布情况及种群特征,从烟台近海盐场采集的土样和水样中利用高氏一号培养基分离得到 22 株中度嗜盐菌.通过菌落形态观察、生理生化特征分析比较,选取其中的 9 株进行 16S rDNA 序列分析和系统进化分析,结果表明这些中度嗜盐菌分别属于7 个属:芽孢杆菌属 ( Bacillus )、枝芽孢杆菌属 ( Virgibacillus ) 、薄壁芽孢杆菌属 ( Gracilibacillus )、刘氏菌属 ( Liuella )、产微球茎菌属 ( Microbulbifer )、拟诺卡氏菌属 ( Nocardiopsis )和交替单胞菌属 ( Alteromonas ),其中菌株YTM-8 、YTM-12 和YTM-13可能是潜在的新种.以上研究结果表明烟台海域中存在有较为丰富的嗜盐微生物多样性,并且潜藏着新类型的嗜盐菌资源.
為研究山東煙檯海域中度嗜鹽菌的分佈情況及種群特徵,從煙檯近海鹽場採集的土樣和水樣中利用高氏一號培養基分離得到 22 株中度嗜鹽菌.通過菌落形態觀察、生理生化特徵分析比較,選取其中的 9 株進行 16S rDNA 序列分析和繫統進化分析,結果錶明這些中度嗜鹽菌分彆屬于7 箇屬:芽孢桿菌屬 ( Bacillus )、枝芽孢桿菌屬 ( Virgibacillus ) 、薄壁芽孢桿菌屬 ( Gracilibacillus )、劉氏菌屬 ( Liuella )、產微毬莖菌屬 ( Microbulbifer )、擬諾卡氏菌屬 ( Nocardiopsis )和交替單胞菌屬 ( Alteromonas ),其中菌株YTM-8 、YTM-12 和YTM-13可能是潛在的新種.以上研究結果錶明煙檯海域中存在有較為豐富的嗜鹽微生物多樣性,併且潛藏著新類型的嗜鹽菌資源.
위연구산동연태해역중도기염균적분포정황급충군특정,종연태근해염장채집적토양화수양중이용고씨일호배양기분리득도 22 주중도기염균.통과균락형태관찰、생리생화특정분석비교,선취기중적 9 주진행 16S rDNA 서렬분석화계통진화분석,결과표명저사중도기염균분별속우7 개속:아포간균속 ( Bacillus )、지아포간균속 ( Virgibacillus ) 、박벽아포간균속 ( Gracilibacillus )、류씨균속 ( Liuella )、산미구경균속 ( Microbulbifer )、의낙잡씨균속 ( Nocardiopsis )화교체단포균속 ( Alteromonas ),기중균주YTM-8 、YTM-12 화YTM-13가능시잠재적신충.이상연구결과표명연태해역중존재유교위봉부적기염미생물다양성,병차잠장착신류형적기염균자원.
To study the distribution and characteristics of moderately halophilic bacteria, the samples were collected from salt field near Yantai sea area in Shandong province. 22 ocean moderately halophilic bacteria strains were isolated on Gause I agar medium. Based on the morphological, physiological and biochemical analysis, the 9 strains were chosen for 16 S rDNA amplification and phylogenetic analysis. Phylogenetic analysis based on 16 S rDNA gene sequences indicated that these moderately halophilic bacteria strains belonged to 7 genera including Liuella, Bacillus, Virgibacillus, Gracilibacillus, Microbulbifer, Nocardiopsis, and Alteromonas respectively. The Strains of YTM-8, YTM-12 and YTM-13 may represent potential new species of their genus. The research results indicate that Yantai sea area is rich in halophilic bacterium diversity and contains new types of microorganism resources.
