CAJ | 학술논문

构建叶片特异表达启动子rbcs驱动的美洲商陆抗病毒蛋白基因PAP-c的植物表达载体PNRPL,冻融法转化农杆菌EHA105后,通过农杆菌介导法侵染甘蔗优良品种ROC22的胚性愈伤组织,经PPT抗性筛选以及PCR鉴定,共获得了24株转化植株,对其中的5株进行Southern杂交检测,有2株呈阳性,初步证明外源基因已整合到甘蔗基因组中;对其接种甘蔗花叶病毒,初步结果表明获得的转化植株对甘蔗花叶病毒具有一定的抗性.
구건협편특이표체계동자rbcs구동적미주상륙항병독단백기인PAP-c적식물표체재체PNRPL,동융법전화농간균EHA105후,통과농간균개도법침염감자우량품충ROC22적배성유상조직,경PPT항성사선이급PCR감정,공획득료24주전화식주,대기중적5주진행Southern잡교검측,유2주정양성,초보증명외원기인이정합도감자기인조중;대기접충감자화협병독,초보결과표명획득적전화식주대감자화협병독구유일정적항성.
Pokeweed antiviral protein gene PAP-c's plant expression vector PNRPL was constructed, which was driven by leaf specific expression promoter rbcs. The Agrobacterium tumerfaciens EHA105 was transformed by the "freeze and thaw" method, and the embryonic calli of elite sugarcane variety ROC22 were inoculated by using the A. tumefaciens -mediated method. PPT resistance screening and PCR identification results showed that 24 transformed plants were generated. Of the transformed plants 5 were taken for Southern blot analysis, 2 of which were positive, preliminarily proving that exogenous gene had been integrated into sugarcanes genome. Inoculation of sugarcane mosaic virus further proved that these transgenic plants had certain resistance on the sugarcane mosaic virus.