时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
3期
546-548
,共3页
陈贤%陆红华%张敏%胡坪%周永传
陳賢%陸紅華%張敏%鬍坪%週永傳
진현%륙홍화%장민%호평%주영전
丹参%丹参总酚酸%大孔吸附树脂%吸附%解吸
丹參%丹參總酚痠%大孔吸附樹脂%吸附%解吸
단삼%단삼총분산%대공흡부수지%흡부%해흡
Salvia mihiorrhiza Bunge%Salvianolic acids%Macroporous resin%Absorption%Desorption
目的 筛选能分离丹参总酚酸的高效、实用的树脂,并优化其吸附分离工艺.方法 以丹酚酸B为指标成分,用紫外分光光度法测定总酚酸含量,考察树脂的吸附、解吸性能.结果 HZ818大孔吸附树脂吸附和解吸性能较好,饱和吸附量为122 mg/g,上样最佳流速为2 BV/h(BV,床层体积),最佳解吸条件为5 BV 50%的乙醇溶液以2.4 BV/h流速进行洗脱,解吸率为93.8%.经HZ818分离得到的丹参总酚酸中丹酚酸B的纯度为65.7%,精制程度达到351.3%,回收率为90.5%.结论 HZ818树脂性能优良,适合分离丹参总酚酸.
目的 篩選能分離丹參總酚痠的高效、實用的樹脂,併優化其吸附分離工藝.方法 以丹酚痠B為指標成分,用紫外分光光度法測定總酚痠含量,攷察樹脂的吸附、解吸性能.結果 HZ818大孔吸附樹脂吸附和解吸性能較好,飽和吸附量為122 mg/g,上樣最佳流速為2 BV/h(BV,床層體積),最佳解吸條件為5 BV 50%的乙醇溶液以2.4 BV/h流速進行洗脫,解吸率為93.8%.經HZ818分離得到的丹參總酚痠中丹酚痠B的純度為65.7%,精製程度達到351.3%,迴收率為90.5%.結論 HZ818樹脂性能優良,適閤分離丹參總酚痠.
목적 사선능분리단삼총분산적고효、실용적수지,병우화기흡부분리공예.방법 이단분산B위지표성분,용자외분광광도법측정총분산함량,고찰수지적흡부、해흡성능.결과 HZ818대공흡부수지흡부화해흡성능교호,포화흡부량위122 mg/g,상양최가류속위2 BV/h(BV,상층체적),최가해흡조건위5 BV 50%적을순용액이2.4 BV/h류속진행세탈,해흡솔위93.8%.경HZ818분리득도적단삼총분산중단분산B적순도위65.7%,정제정도체도351.3%,회수솔위90.5%.결론 HZ818수지성능우량,괄합분리단삼총분산.
Objective To obtain an efficient macroporous resin for salvianolic acids absorption and desorption, and to optimize the method for salvianolic acids separation. Methods The concentration of salvianolic acid B was measured by UV spectrophotometry. The absorption and desorption properties of resins were studied. Results The absorption ability of HZ818 resin was 122 mg/g, the best loading flow rate was 2 BV/h (BV, bed volume), the best desorption rate was 93.8% with conditions of 5 BV of 50% ethanol as a elution and the desorption flow rate was 2.4 BV/h. The purity of salvianolic acid B was 65.7% with 351.3% purification factor and 90.5% recovery.Conclusion The resin of HZ818 was suitable for purification of salvianolic acids.
