CAJ | 학술논문

目的探讨14-3-3σ基因与乳腺癌发生的相关性.方法采用甲基化特异性聚合酶链反应法对散发性乳腺癌患者癌组织标本进行14-3-3σ甲基化检测,SYBR green Ⅰ实时定量PCR法检测14-3-3σmRNA转录水平.结果采用相对量分析法,△△CT为0.77,根据样本平均相对含量(%)=2~(-△△CT),得出14-3-3σ基因在癌症组平均转录表达量是非癌组的58%.14-3-3σ基因启动子在42例散发性乳腺癌标本中甲基化率为66.7%,在24例乳腺非癌组织中甲基化率为25%,二者差异有显著性(χ~2 =10.616, P <0.05).结论 14-3-3σ基因在乳腺癌组织中有较高的甲基化率,且mRNA表达水平降低,可能甲基化的异常影响了14-3-3σ基因的表达,从而促进乳腺癌的发生.
목적 탐토14-3-3σ기인여유선암발생적상관성.방법 채용갑기화특이성취합매련반응법대산발성유선암환자암조직표본진행14-3-3σ갑기화검측,SYBR green Ⅰ실시정량PCR법검측14-3-3σmRNA전록수평.결과 채용상대량분석법,△△CT위0.77,근거양본평균상대함량(%)=2~(-△△CT),득출14-3-3σ기인재암증조평균전록표체량시비암조적58%.14-3-3σ기인계동자재42례산발성유선암표본중갑기화솔위66.7%,재24례유선비암조직중갑기화솔위25%,이자차이유현저성(χ~2 =10.616, P <0.05).결론 14-3-3σ기인재유선암조직중유교고적갑기화솔,차mRNA표체수평강저,가능갑기화적이상영향료14-3-3σ기인적표체,종이촉진유선암적발생.
Objective To study the methylation and mRNA expression of 14-3-3σ gene in sporadic breast cancer and to investigate the correlation between 14-3-3σ gene and breast cancer. Methods The methylation of 14-3-3σ gene in breast cancer tissues was examined by methylation-specific polymerase chain reaction (MSP). The mRNA expression was assessed using SYBR green I real time PCR. Results The average expression level of 14-3-3σ gene in cancer tissue was 58% in relative amount analysis. Methylation rates of 14-3-3σ gene in cancer group and control group were 66.7% and 25% (χ~2 =10.616, P <0.05),respectively. Conclusion Higher methylation rate and lower expression level of 14-3-3σ gene occur in breast cancer. Abnomal methylation of 14-3-3σ gene may affect its expression and promote breast carcinogenesis