国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
17期
1310-1313
,共4页
非小细胞肺癌%P53蛋白%呼出气冷凝液
非小細胞肺癌%P53蛋白%呼齣氣冷凝液
비소세포폐암%P53단백%호출기냉응액
NSCLC%Protein P53%Exhaled breath condensate
目的 研究非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)患者肿瘤组织及呼出气冷凝液(exhaled breath condensate,EBC)中P53蛋白的表达及其临床意义.方法 收集NSCLC患者肿瘤组织和EBC,采用免疫组织化学方法(SP)检测肿瘤组织及对照正常肺组织中的P53蛋白表达水平,采用酶免疫法检测肺癌组及对照组EBC中P53蛋白的水平.结果 NSCLC患者肿瘤组织中P53蛋白表达阳性率为60.7％,明显高于对照组(P＜0.01)；不同病理类型及不同病理分期肿瘤组织中P53蛋白表达无显著性差异；NSCLC患者EBC中P53蛋白浓度为(50.81±14.23) ng/L,明显较正常对照组高(P＜0.01)；不同病理类型NSCLC患者EBC中P53蛋白浓度无显著性差异,Ⅱ+Ⅲ+Ⅳ期NSCLC患者EBC中P53蛋白浓度较Ⅰ期高；肺癌组肿瘤组织中P53蛋白检测的敏感性和特异性为60.7％和90.0％,EBC中为42.4％和93.4％,两种检测途径无显著性差异(P＜0.05).结论 NSCLC患者肿瘤组织及EBC中P53蛋白检测可用于肺癌的辅助诊断及病情监测.
目的 研究非小細胞肺癌(nonsmall-cell lung cancer,NSCLC)患者腫瘤組織及呼齣氣冷凝液(exhaled breath condensate,EBC)中P53蛋白的錶達及其臨床意義.方法 收集NSCLC患者腫瘤組織和EBC,採用免疫組織化學方法(SP)檢測腫瘤組織及對照正常肺組織中的P53蛋白錶達水平,採用酶免疫法檢測肺癌組及對照組EBC中P53蛋白的水平.結果 NSCLC患者腫瘤組織中P53蛋白錶達暘性率為60.7％,明顯高于對照組(P＜0.01)；不同病理類型及不同病理分期腫瘤組織中P53蛋白錶達無顯著性差異；NSCLC患者EBC中P53蛋白濃度為(50.81±14.23) ng/L,明顯較正常對照組高(P＜0.01)；不同病理類型NSCLC患者EBC中P53蛋白濃度無顯著性差異,Ⅱ+Ⅲ+Ⅳ期NSCLC患者EBC中P53蛋白濃度較Ⅰ期高；肺癌組腫瘤組織中P53蛋白檢測的敏感性和特異性為60.7％和90.0％,EBC中為42.4％和93.4％,兩種檢測途徑無顯著性差異(P＜0.05).結論 NSCLC患者腫瘤組織及EBC中P53蛋白檢測可用于肺癌的輔助診斷及病情鑑測.
목적 연구비소세포폐암(nonsmall-cell lung cancer,NSCLC)환자종류조직급호출기냉응액(exhaled breath condensate,EBC)중P53단백적표체급기림상의의.방법 수집NSCLC환자종류조직화EBC,채용면역조직화학방법(SP)검측종류조직급대조정상폐조직중적P53단백표체수평,채용매면역법검측폐암조급대조조EBC중P53단백적수평.결과 NSCLC환자종류조직중P53단백표체양성솔위60.7％,명현고우대조조(P＜0.01)；불동병리류형급불동병리분기종류조직중P53단백표체무현저성차이；NSCLC환자EBC중P53단백농도위(50.81±14.23) ng/L,명현교정상대조조고(P＜0.01)；불동병리류형NSCLC환자EBC중P53단백농도무현저성차이,Ⅱ+Ⅲ+Ⅳ기NSCLC환자EBC중P53단백농도교Ⅰ기고；폐암조종류조직중P53단백검측적민감성화특이성위60.7％화90.0％,EBC중위42.4％화93.4％,량충검측도경무현저성차이(P＜0.05).결론 NSCLC환자종류조직급EBC중P53단백검측가용우폐암적보조진단급병정감측.
Objective To investigate the clinical significance of detecting P53 protein in tumor tissue and exhaled breath condensate (EBC) of NSCLC patients.Methods To collected tumor tissue and EBC of NSCLC patients,expression of P53 protein in tumor tissue and normal tissue were evaluated by immunohistochemistry,Level of P53 protein in EBC were detected by enzyme linked immunosorbent assay method.Results P53 protein's expression positive rate in NSCLC patients tumor tissues were 60.7％,it was significantly higher than normal control group ( P ＜ 0.01 ) and had no significant difference in different types and TNM stages.The levels of P53 protein in NSCLC group's EBC was (50.81 ± 14.23) ng/L,it was significantly higher than normal control group ( P ＜0.01).it had no significant difference in different types ( P ＞0.05) and in Ⅱ + Ⅲ + Ⅳ stage were significantly higher than in Ⅰ stage ( P ＜0.05).The sensitivity and specificity of P53 protein in tumor tissue detection pathway were 60.7％and 90.0％,when in EBC detection pathway were 42.4％ and 93.4％,there were no significant difference in twe pathways ( P ＜0.05).Conclusions Detecting P53 protein's level in tumor tissue aud EBC was helpful to diagnosis,evaluation of illness state of NSCLC patients.
