中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
12期
1071-1073
,共3页
闫丽萍%易建良%徐恒泽%李守栋%马骋
閆麗萍%易建良%徐恆澤%李守棟%馬騁
염려평%역건량%서항택%리수동%마빙
神经病理性痛%电针%脊髓%脊髓谷氨酸%NMDA受体
神經病理性痛%電針%脊髓%脊髓穀氨痠%NMDA受體
신경병이성통%전침%척수%척수곡안산%NMDA수체
Neuropathic pain%Electroacupuncture%Spinal cord%Glu%NMDAR
目的 探讨电针对神经病理性痛大鼠痛觉过敏及其脊髓谷氨酸(Glu)与NMDA R1表达的影响.方法 雄性SD大鼠,逐一行基础痛阈测定,剔除痛阈过高和过低者后,随机分出假手术组(n=16)和模型动物组.模型动物组实施慢性坐骨神经缩窄损伤(CCI)手术.待模型成功后将模型动物随机分为手术组和电针组(n=16).电针"委中"和"环跳"穴,观察其对大鼠机械痛阈和热痛阈的影响,并以OPA柱前衍生+HPLC荧光检测及免疫组化等方法分别测定脊髓Glu水平以及背角Glu和NMAR1阳性表达的变化.结果 CCI手术可明显降低大鼠机械痛阈和热痛阈,手术组10d时分别为(12.10±2.25)g和(10.09±1.23)s,与假手术组相比,差异有显著性(P<0.01);并且手术组脊髓Glu水平明显升高,为(23.66±4.37)μmoL/mg,其损伤侧背角Glu阳性终末表达减少(0.16±0.01),NMAR1的阳性表达升高(0.12±0.03),与假手术组相比,均差异有显著性(P<0.01,P<0.05);电针连续7次十预后,脊髓Glu水平以及背角Glu终末和NMAR1阳性表达均被逆转,与手术组各项比较差异有显著性(P<0.01,P<0.05);与此同时明显改善CCI大鼠的痛敏状态与痛行为.结论 电针减轻神经病理性痛的脊髓机制之一,可能与其有效地抑制大鼠脊髓相应节段Glu的释放、调整NMDA受体的功能状态有关.
目的 探討電針對神經病理性痛大鼠痛覺過敏及其脊髓穀氨痠(Glu)與NMDA R1錶達的影響.方法 雄性SD大鼠,逐一行基礎痛閾測定,剔除痛閾過高和過低者後,隨機分齣假手術組(n=16)和模型動物組.模型動物組實施慢性坐骨神經縮窄損傷(CCI)手術.待模型成功後將模型動物隨機分為手術組和電針組(n=16).電針"委中"和"環跳"穴,觀察其對大鼠機械痛閾和熱痛閾的影響,併以OPA柱前衍生+HPLC熒光檢測及免疫組化等方法分彆測定脊髓Glu水平以及揹角Glu和NMAR1暘性錶達的變化.結果 CCI手術可明顯降低大鼠機械痛閾和熱痛閾,手術組10d時分彆為(12.10±2.25)g和(10.09±1.23)s,與假手術組相比,差異有顯著性(P<0.01);併且手術組脊髓Glu水平明顯升高,為(23.66±4.37)μmoL/mg,其損傷側揹角Glu暘性終末錶達減少(0.16±0.01),NMAR1的暘性錶達升高(0.12±0.03),與假手術組相比,均差異有顯著性(P<0.01,P<0.05);電針連續7次十預後,脊髓Glu水平以及揹角Glu終末和NMAR1暘性錶達均被逆轉,與手術組各項比較差異有顯著性(P<0.01,P<0.05);與此同時明顯改善CCI大鼠的痛敏狀態與痛行為.結論 電針減輕神經病理性痛的脊髓機製之一,可能與其有效地抑製大鼠脊髓相應節段Glu的釋放、調整NMDA受體的功能狀態有關.
목적 탐토전침대신경병이성통대서통각과민급기척수곡안산(Glu)여NMDA R1표체적영향.방법 웅성SD대서,축일행기출통역측정,척제통역과고화과저자후,수궤분출가수술조(n=16)화모형동물조.모형동물조실시만성좌골신경축착손상(CCI)수술.대모형성공후장모형동물수궤분위수술조화전침조(n=16).전침"위중"화"배도"혈,관찰기대대서궤계통역화열통역적영향,병이OPA주전연생+HPLC형광검측급면역조화등방법분별측정척수Glu수평이급배각Glu화NMAR1양성표체적변화.결과 CCI수술가명현강저대서궤계통역화열통역,수술조10d시분별위(12.10±2.25)g화(10.09±1.23)s,여가수술조상비,차이유현저성(P<0.01);병차수술조척수Glu수평명현승고,위(23.66±4.37)μmoL/mg,기손상측배각Glu양성종말표체감소(0.16±0.01),NMAR1적양성표체승고(0.12±0.03),여가수술조상비,균차이유현저성(P<0.01,P<0.05);전침련속7차십예후,척수Glu수평이급배각Glu종말화NMAR1양성표체균피역전,여수술조각항비교차이유현저성(P<0.01,P<0.05);여차동시명현개선CCI대서적통민상태여통행위.결론 전침감경신경병이성통적척수궤제지일,가능여기유효지억제대서척수상응절단Glu적석방、조정NMDA수체적공능상태유관.
Objective To investigate the effect of electroacupuncture(EA) on hyperalgia and glutamic acid (Glu) and expression of NMDA receptor in rats with neuropathic pain. Methods SD rats, healthy and male, were tested the base mechanical pain threshold by dynamic plantar aesthesiometer. After removed the rats with high or low pain threshold, randomly divided into sham operation group ( n = 16 ) and model group ( n = 32) with chronic constriction injury of the sciatic nerve(CCI). After the CCI model of rats was established, the rats divided into model group( n= 16) and EA group( n= 16) continusly. The CCI rats were treated by EA (2Hz, onset 1mA, enhance 1mA each 10 min) on Huan tiao ( GB 30) and Wei zhong ( BL 40) , and the effects of EA was observed by tested the mechanical and thermal pain thresholds. The contents of Glu in spinal cord was tested by HPLC with OPA derivatized, and the expressions of Glu positive neurons, the NR1 and NR2B receptor subunits were measured by immunohistochemistry. Results At 10th day, the mechanical and thermal pain thresholds were reduced on CCI rats markedly, and showed (12.10 ±2.25)g and ( 10.09 ± 1.23) s respectively. There were significantly difference compared with the sham operation group (P<0.01).The contents of Glu was significantly increased (23.66 ±4.37)μmol/mg, and the expressions of Glu positive neurons was reduced markedly in spinal cord(0.16 ±0.01 ) , the significant difference was existed. The expressions of NR1 also increased in spinal cord (0.12 ±0:03) , and existed significantly difference compared with the sham operation group (P < 0.05). After CCI rats treated by 2Hz EA, 7 day, the contents of Glu, the expressions of Glu positive neurons, and NR1 subunits were all significantly reversed in rats with CCI(P<0.01, P<0.05). Same times, the behavior of pain was improved. Conclusion EA could efficaciously inhibited releasing of Glu, and adjusted the function and the conditions of NMDA receptor in related segment of spinal cord. It is the analgesia mechanisms of EA on neuropathic pain in spinal cord.