CAJ | 학술논문

目的探讨核心蛋白聚糖(DCN)在移植肝慢性排斥反应(CR)患者肝组织及血清中的表达及意义.方法以免疫组织化学(SP法)检测正常人16例、肝硬化20例、移植肝无CR 46例及合并CR 8例患者肝组织中DCN的表达情况,以酶联免疫方法(Elisa法)测定各组血清中DCN的含量情况.结果移植肝并CR与无CR组织及正常组织中DCN均呈阴性表达,肝硬化组织中呈阳性表达,移植肝并CR阳性表达率、平均吸光度与肝硬化组织相比,有显著性差异(25%vs 55%,0.1249±0.0039 vs 0.2357±0.0396,P<0.01),与正常肝及移植肝无CR组相比,差异无统计学意义;移植肝并CR患者血清中DCN含量明显升高,与正常人、肝硬化及移植肝无CR组相比,有显著性差异(54.0833±6.0325μg/L vs 1.0232±0.9105 μg/L,12.6202±1.5370μg/L,17.7102±2.3562 μg/L,P<0.01),DCN血清浓度与排斥反应程度呈正相关.结论 DCN在移植肝并CR患者肝组织中呈阴性表达,而在血清中呈明显升高,提示其可能参与CR的发生、发展,同时血清DCN测定有可能成为移植肝CR早期诊断、发展及预后的非创伤性重要指标.
목적 탐토핵심단백취당(DCN)재이식간만성배척반응(CR)환자간조직급혈청중적표체급의의.방법 이면역조직화학(SP법)검측정상인16례、간경화20례、이식간무CR 46례급합병CR 8례환자간조직중DCN적표체정황,이매련면역방법(Elisa법)측정각조혈청중DCN적함량정황.결과 이식간병CR여무CR조직급정상조직중DCN균정음성표체,간경화조직중정양성표체,이식간병CR양성표체솔、평균흡광도여간경화조직상비,유현저성차이(25%vs 55%,0.1249±0.0039 vs 0.2357±0.0396,P<0.01),여정상간급이식간무CR조상비,차이무통계학의의;이식간병CR환자혈청중DCN함량명현승고,여정상인、간경화급이식간무CR조상비,유현저성차이(54.0833±6.0325μg/L vs 1.0232±0.9105 μg/L,12.6202±1.5370μg/L,17.7102±2.3562 μg/L,P<0.01),DCN혈청농도여배척반응정도정정상관.결론 DCN재이식간병CR환자간조직중정음성표체,이재혈청중정명현승고,제시기가능삼여CR적발생、발전,동시혈청DCN측정유가능성위이식간CR조기진단、발전급예후적비창상성중요지표.
Objective To investigate expression and significance of decorin(DCN)in liver tissue and serum of liver transplant patients with chronic rejection(CR).Methods Immunohistochemistry(SP method)was used to detect expression of DCN in liver tissue of 16 normal controls, 20 patients with cirrhosis, 46 liver translantion patients without CR and 8 patients with CR.Enzyme-linked immunosorbent assay method(ELISA method)was used to determined the content of DCN in serum of all research subjects.Results The expression of DCN was negative in normal hepatic tissues and with/without CR, cirrhosis tissues showed strong expression of DCN.The positive expression rate and the average optical density value of DCN in liver transplant tissues with CR had significant difference comparing with Cirrhosis tissues(25% vs 55%, 0.1249 ±0.0039 vs 0.2357 ±0.0396, P <0.01,while no statistic siqnificance compared to normal liver tissues and those without CR.The level of DCN in serum was significantly higher in liver transplant patients with CR, with significant difference comparing with normal people, liver cirrhosis and transplant liver patients without CR(54.0833 ± 6.0325)μg/L vs(1.0232 ± 0.9105)μg/L,(12.6202 ± 1.5370)μg/L,(17.7102 ± 2.3562)μg/L, P < 0.01).The concentration of DCN in serum showed a positive correlation with the degree of CR.Conclusions DCN showed negative expression in liver tissue and increased significantly in serum of liver transplantation patients with CR.This suggests that DCN may be involved in occurrence and development of CR.At the same time the determination of DCN in serum maybe become an important indicator of the early diagnosis, development and prognosis of CR for liver transplant patients.