中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
5期
388-390
,共3页
王寿平%陈晓彤%苗利萍%彭书崚
王壽平%陳曉彤%苗利萍%彭書崚
왕수평%진효동%묘리평%팽서릉
异氟醚%胎儿%凋亡%脑发育
異氟醚%胎兒%凋亡%腦髮育
이불미%태인%조망%뇌발육
Isoflurane%Fetus%Apoptosis%Brain development
目的 本研究探讨不同浓度的异氟醚对胎鼠海马区Caspase-3表达及血浆S100β水平的影响.方法 18只健康清洁级SD孕21 d大鼠随机数字表法分成对照组(C组)、1.3%异氟醚组(1.3% ISO组)、3%异氟醚组(3%ISO组).C组不给予任何药物,1.3%ISO、3%ISO组分别吸入1.3%、3%异氟醚1 h,术中呼吸机控制呼吸,持续动态监测动脉血压,在模型建立完毕时(基础值)、30min后、1 h时抽取股动脉血检测血糖、血气.6h后3组孕鼠开腹随机取两只胎鼠,抽取胎鼠血液行S100β检测、通过免疫组化检测胎鼠海马CA1区Caspase-3阳性标记神经元的方法判断凋亡的情况.结果 与对照组胎鼠血浆S100β[(1.48±0.08)μg/L]比较,1.3%ISO组胎鼠[(1.53±0.12)μg/L]稍有升高,但两者间的差异无统计学意义,3%ISO组胎鼠[(3.12±0.15)μg/L,P＜0.05]明显升高,差异有统计学意义.与Con组胎鼠海马CA1区Caspase-3阳性标记的神经元数量比较[(33±4)个/mm2],1.3%ISO组胎鼠无明显改变[(31±5)爪/mm2],3%ISO组胎鼠明显增多[(75±7)个/mm2,P＜0.05],差异有统计学意义.结论 异氟醚对胎鼠脑发育的影响存在浓度依赖性,宫内暴露于3%异氟醚1h可诱发脑损伤,导致胎鼠血浆S100β浓度升高、诱发海马CA1区神经元发生凋亡;暴露于低浓度1.3%异氟醚1h不足以诱发脑损伤.
目的 本研究探討不同濃度的異氟醚對胎鼠海馬區Caspase-3錶達及血漿S100β水平的影響.方法 18隻健康清潔級SD孕21 d大鼠隨機數字錶法分成對照組(C組)、1.3%異氟醚組(1.3% ISO組)、3%異氟醚組(3%ISO組).C組不給予任何藥物,1.3%ISO、3%ISO組分彆吸入1.3%、3%異氟醚1 h,術中呼吸機控製呼吸,持續動態鑑測動脈血壓,在模型建立完畢時(基礎值)、30min後、1 h時抽取股動脈血檢測血糖、血氣.6h後3組孕鼠開腹隨機取兩隻胎鼠,抽取胎鼠血液行S100β檢測、通過免疫組化檢測胎鼠海馬CA1區Caspase-3暘性標記神經元的方法判斷凋亡的情況.結果 與對照組胎鼠血漿S100β[(1.48±0.08)μg/L]比較,1.3%ISO組胎鼠[(1.53±0.12)μg/L]稍有升高,但兩者間的差異無統計學意義,3%ISO組胎鼠[(3.12±0.15)μg/L,P＜0.05]明顯升高,差異有統計學意義.與Con組胎鼠海馬CA1區Caspase-3暘性標記的神經元數量比較[(33±4)箇/mm2],1.3%ISO組胎鼠無明顯改變[(31±5)爪/mm2],3%ISO組胎鼠明顯增多[(75±7)箇/mm2,P＜0.05],差異有統計學意義.結論 異氟醚對胎鼠腦髮育的影響存在濃度依賴性,宮內暴露于3%異氟醚1h可誘髮腦損傷,導緻胎鼠血漿S100β濃度升高、誘髮海馬CA1區神經元髮生凋亡;暴露于低濃度1.3%異氟醚1h不足以誘髮腦損傷.
목적 본연구탐토불동농도적이불미대태서해마구Caspase-3표체급혈장S100β수평적영향.방법 18지건강청길급SD잉21 d대서수궤수자표법분성대조조(C조)、1.3%이불미조(1.3% ISO조)、3%이불미조(3%ISO조).C조불급여임하약물,1.3%ISO、3%ISO조분별흡입1.3%、3%이불미1 h,술중호흡궤공제호흡,지속동태감측동맥혈압,재모형건립완필시(기출치)、30min후、1 h시추취고동맥혈검측혈당、혈기.6h후3조잉서개복수궤취량지태서,추취태서혈액행S100β검측、통과면역조화검측태서해마CA1구Caspase-3양성표기신경원적방법판단조망적정황.결과 여대조조태서혈장S100β[(1.48±0.08)μg/L]비교,1.3%ISO조태서[(1.53±0.12)μg/L]초유승고,단량자간적차이무통계학의의,3%ISO조태서[(3.12±0.15)μg/L,P＜0.05]명현승고,차이유통계학의의.여Con조태서해마CA1구Caspase-3양성표기적신경원수량비교[(33±4)개/mm2],1.3%ISO조태서무명현개변[(31±5)조/mm2],3%ISO조태서명현증다[(75±7)개/mm2,P＜0.05],차이유통계학의의.결론 이불미대태서뇌발육적영향존재농도의뢰성,궁내폭로우3%이불미1h가유발뇌손상,도치태서혈장S100β농도승고、유발해마CA1구신경원발생조망;폭로우저농도1.3%이불미1h불족이유발뇌손상.
Objective To investigate the effects of different concentrations of isoflurane on the caspase-3 expression in the hippocampus and S100β level of plasma in fetal rats. Methods 18 pregnant rats at gestational day 21 were divided into control group, 1. 3% isoflurane group,3% isoflurane group. Rats in the control group spontaneously breathed 100% oxygen for 1 h. Rats in the treatment groups breathed 1.3% or 3% isoflurane in 100% oxygen through an endotracheal tube, with mechanical ventilation for 1 h. Rat pups were delivered by cesarean section 6 h after treatment, and fetal blood was sampled from the left ventricle of each fetal heart and evaluated for S100β. Fetal brains were then evaluated for apoptosis, using caspase-3 immunohistochemistry in the CA1 region of the hippocampus. Results Compared to the control group ((1. 48 ± 0. 08) μg/L) and the 1. 3% isoflurane group( (1.53 ±0. 12)μg/L) ,the 3% isoflurane group showed significantly higher level of S100β( (3. 12 ±0. 15) μg/L, P＜0.05) . There was no differences in densities of caspase-3-positive cells between the control ((33 ±4) cell/mm ) and 1.3% isoflurane groups((31 ±5)cell/mm2). Compared to 1.3% isoflurane,isoflurane at a concentration of 3%((75 ± 7) cell/mm2, P＜0.05) for lh increased neurodegeneration in the hippocampal CA1 area in the developing brain of fetal rats. Conclusion Isoflurane can dose-dependently induce brain damage. Isoflurane at a concentration of 3% for lh can induce apoptosis in the hippocampal CA1 area and increase S100β levels of fetal rats.
