CAJ | 학술논문

目的探讨心理应激复合睡眠剥夺大鼠血清超氧化物歧化酶(SOD)、丙二醛(MDA)、总抗氧化能力(T-AOC)和谷胱甘肽-过氧化物酶(GSH-PX)含量的变化.方法将105只雄性SD大鼠,随机分为空白对照组、心理应激单因素组、心理应激阴性对照组、睡眠剥夺48 h单因素组、睡眠剥夺阴性对照组、心理应激14 d+睡眠剥夺48 h复合因素组和复合因素阴性对照组,每组15只.采用Communication Box建立心理应激模型.采用改良后的小平台法睡眠剥夺模型进行睡眠剥夺.所有动物取血清1.0 ml测定MDA含量、SOD、GSH-PX和T-AOC活力.结果与正常对照组相比,心理应激组大鼠血清SOD活力、GSH.PX活力增加(P<0.05),T-AOC活力有增加趋势,MDA含量有降低趋势;睡眠剥夺组SOD活力增加(P<0.05)、T-AOC活力有增加趋势,GSH-PX活力、MDA含量有降低趋势;心理应激复合睡眠剥夺组SOD活力及MDA含量增加(P<0.05),T-AOC活力有增加趋势,GSH-PX活力降低(P<0.05).与心理应激和睡眠剥夺单一因素组相比,复合因素组MDA含量和T-AOC活力高于单一因素组(P<0.05),GSH-PX活力有降低趋势,SOD活力高于心理应激组,低于睡眠剥夺组.结论心理应激、睡眠剥夺及心理应激复合睡眠剥夺后,大鼠的抗氧化能力提高,复合因素组高于单一因素组.
목적 탐토심리응격복합수면박탈대서혈청초양화물기화매(SOD)、병이철(MDA)、총항양화능력(T-AOC)화곡광감태-과양화물매(GSH-PX)함량적변화.방법 장105지웅성SD대서,수궤분위공백대조조、심리응격단인소조、심리응격음성대조조、수면박탈48 h단인소조、수면박탈음성대조조、심리응격14 d+수면박탈48 h복합인소조화복합인소음성대조조,매조15지.채용Communication Box건립심리응격모형.채용개량후적소평태법수면박탈모형진행수면박탈.소유동물취혈청1.0 ml측정MDA함량、SOD、GSH-PX화T-AOC활력.결과 여정상대조조상비,심리응격조대서혈청SOD활력、GSH.PX활력증가(P<0.05),T-AOC활력유증가추세,MDA함량유강저추세;수면박탈조SOD활력증가(P<0.05)、T-AOC활력유증가추세,GSH-PX활력、MDA함량유강저추세;심리응격복합수면박탈조SOD활력급MDA함량증가(P<0.05),T-AOC활력유증가추세,GSH-PX활력강저(P<0.05).여심리응격화수면박탈단일인소조상비,복합인소조MDA함량화T-AOC활력고우단일인소조(P<0.05),GSH-PX활력유강저추세,SOD활력고우심리응격조,저우수면박탈조.결론 심리응격、수면박탈급심리응격복합수면박탈후,대서적항양화능력제고,복합인소조고우단일인소조.
Objective To investigate effects of psychological stress combined with sleep deprivation on levels of supemxide dismutase (SOD),glutathione-superoxide(GSH-PX),T-AOC and malondialdehyde (MDA) in rats.Methods one hundred and five male SD rats were randomly divided into 7 groups,each consisting of 15 rats:the control group,the psychological stress group,the psychological stress control group,the sleep deprivation group,the sleep deprivation controt group,the psychological stress combined with sleep deprivation group and the multi-factor control group.The psychological stress model was developed with the Communication Box.Rats were deprived of sleep with the application of a small modified platform.one point zero ml of serum was taken from all the sacrificed animals to measure SOD.GsH-PX,MDA levels and T-AOC activity.Results Compared with those of the control group,SOD and GSH-PX activity increased (P<0.05), T-AOC activity had a tendency to increase.while MDA concentration showed a tendency to decrease in the psychological stress group.SOD activity of the sleep deprivation group increased (P<0.05),the T-AOC activity had a tendency to increase,while GSH-PX activity and MDA concentration showed a tendency to decrease.SOD activity and MDA level of the psychological stress combined with sleep deprivation group increased(P<0.05),the T-AOC activity showed a tendency to increase,while GSH-PX activity diminished obviously (P<0.05).Compared with the psychological stress and sleep deprivation single-factor groups,TAOC and MDA of the multi-factor group were higher than those of the single-factor group (P<0.05).The activity of GSH-PX showed a tendency to decrease,and the activity of SOD was higher than that of the psychological stress group,but lower than that of the sleep deprivation group.Conclusions Antioxidation in rats improved,following psychological stress,sleep deprivation and psychological stress combined with sleep deprivation,with the antioxidative capacity of the multi-factor group being higher than that of the single-factor group.