CAJ | 학술논문

目的探讨吉西他滨(GEM)是否对非小细胞肺癌具有放射增敏作用,并对GEM的放射增敏机制进行初步探讨.方法用克隆形成分析法观察GEM对p53基因突变的人肺腺癌细胞系(973细胞)的放射增敏效应.流式细胞术观察照射前后973细胞周期分布和细胞凋亡,分析其与p53基因突变是否为放射增敏机制.结果 10 nmol/L GEM照前、照后给药均具有极轻微放射增敏作用;100 nmol/L GEM照前、照后给药时均具有明显放射增敏作用,且照前给药组的增敏作用明显强于照后给药组.p53基因突变影响细胞周期再分布及细胞凋亡,但与GEM的放射增敏作用无关.结论 100 nmol/L GEM具有明显放射增敏作用,p53基因突变、细胞周期再分布及细胞凋亡不是GEM放射增敏作用的主要机制.
목적 탐토길서타빈(GEM)시부대비소세포폐암구유방사증민작용,병대GEM적방사증민궤제진행초보탐토.방법 용극륭형성분석법관찰GEM대p53기인돌변적인폐선암세포계(973세포)적방사증민효응.류식세포술관찰조사전후973세포주기분포화세포조망,분석기여p53기인돌변시부위방사증민궤제.결과 10 nmol/L GEM조전、조후급약균구유겁경미방사증민작용;100 nmol/L GEM조전、조후급약시균구유명현방사증민작용,차조전급약조적증민작용명현강우조후급약조.p53기인돌변영향세포주기재분포급세포조망,단여GEM적방사증민작용무관.결론 100 nmol/L GEM구유명현방사증민작용,p53기인돌변、세포주기재분포급세포조망불시GEM방사증민작용적주요궤제.
Objective To investigate whether gemcitabine (GEM) could enhance radiosensitivity of human non-small cell lung cancer cells and its related mechanism.Methods Clonogenic assay was used to analyze radiosensitivity enhancement by GEM on p53 mutant human lung adenocarcinoma cell line 973.Alterations of cell cycle distribution and apoptosis were measured by flow cytometry.Results Mild radiosesitizing effect was observed when 10 nmol/L GEM was administrated before or after irradiation.Marked radiosesitizing effect was demonstrated when 100 nmol/L GEM was administrated before or after irradiation, with much stronger effect of pre-irradiation GEM treatment.Mutation of p53 gene affected cell cycle redistribution and cell apoptosis, but had no relationship with radiosensitivity enhancement of GEM.Conclusions 100 nmol/L GEM could significantly enhance radiosensitivity of human lung cancer cells.However, this effect may not be associated with p53 gene mutation, cell cycle redistribution or cell apoptosis.