中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
4期
234-237
,共4页
张馨予%陈集敏%梁伶%曹存巍%刘栋华%黄绍标%刘燕芬%严煜林%李菊裳
張馨予%陳集敏%樑伶%曹存巍%劉棟華%黃紹標%劉燕芬%嚴煜林%李菊裳
장형여%진집민%량령%조존외%류동화%황소표%류연분%엄욱림%리국상
青霉属%抗真菌药%微生物敏感性试验
青黴屬%抗真菌藥%微生物敏感性試驗
청매속%항진균약%미생물민감성시험
Penicillium%Antifungal agents%Microbial sensitivity tests
目的 观察马尔尼菲青霉(PM)广西野生银星竹鼠寄生株与临床人分离株对伏立康唑和几种常用抗真菌药物的敏感性.方法 采用美国临床实验室标准委员会( CLSI )M27-A2和M38-A方案中的微量稀释法,测定伏立康唑、伊曲康唑、特比萘芬、两性霉素B及氟康唑对14株广西野生银星竹鼠PM寄生株与25株临床人PM分离株25℃菌丝相及37 ℃酵母相的最小抑菌浓度(MIC).用两样本均数比较t检验比较PM寄生株与临床人PM分离株MIC的差异性,用配对t检验比较同一株菌在两种不同温度相下的MIC差异性.结果 伏立康唑、伊曲康唑、特比萘芬、两性霉素B、氟康唑对PM寄生株菌丝相的MIC分别为:0.0313 ～ 0.1250、0.1250 ～ 1.0000、0.0313 ～ 0.5000、0.2500 ～ 4.0000、2.0000 ～ 8.0000 mg/L;对PM寄生株酵母相的MIC分别为:0.0078 ～ 0.2500、0.0313～ 0.5000、0.0313 ～ 1.0000、0.2500～2.0000、1.0000～8.0000 mg/L;对PM临床人分离株菌丝相的MIC分别为:0.0313～0.2500、0.0625 ～ 1.0000、0.0313～1.0000、0.2500～4.0000、2.0000～32.0000 mg/L;对PM临床人分离株酵母相的MIC分别为:0.0039～0.2500、0.0313 ～ 0.5000、0.0313～ 2.0000、0.1250 ～ 2.0000、2.0000 ～ 16.0000mg/L.5种抗真菌药物对广西野生银星竹鼠PM寄生株与临床人PM分离株菌丝相和酵母相均敏感.同一温度下伏立康唑对两种不同来源PM的MIC最低,其他药物的MIC依次为伊曲康唑、特比萘芬＜两性霉素B＜氟康唑.同一药物在同一温度下对广西野生银星竹鼠PM寄生株与PM临床分离株的MIC无明显差异;伊曲康唑、两性霉素B、特比萘芬对同一菌株在菌丝相和酵母相下的MIC存在差异.结论 PM对伏立康唑的敏感性最高;来自于广西野生银星竹鼠的PM寄生株与临床人PM分离株对伏立康唑、伊曲康唑、特比萘芬、两性霉素B及氟康唑的MIC类似;菌相的改变可影响PM对伊曲康唑、两性霉素B、特比萘芬的敏感性.
目的 觀察馬爾尼菲青黴(PM)廣西野生銀星竹鼠寄生株與臨床人分離株對伏立康唑和幾種常用抗真菌藥物的敏感性.方法 採用美國臨床實驗室標準委員會( CLSI )M27-A2和M38-A方案中的微量稀釋法,測定伏立康唑、伊麯康唑、特比萘芬、兩性黴素B及氟康唑對14株廣西野生銀星竹鼠PM寄生株與25株臨床人PM分離株25℃菌絲相及37 ℃酵母相的最小抑菌濃度(MIC).用兩樣本均數比較t檢驗比較PM寄生株與臨床人PM分離株MIC的差異性,用配對t檢驗比較同一株菌在兩種不同溫度相下的MIC差異性.結果 伏立康唑、伊麯康唑、特比萘芬、兩性黴素B、氟康唑對PM寄生株菌絲相的MIC分彆為:0.0313 ～ 0.1250、0.1250 ～ 1.0000、0.0313 ～ 0.5000、0.2500 ～ 4.0000、2.0000 ～ 8.0000 mg/L;對PM寄生株酵母相的MIC分彆為:0.0078 ～ 0.2500、0.0313～ 0.5000、0.0313 ～ 1.0000、0.2500～2.0000、1.0000～8.0000 mg/L;對PM臨床人分離株菌絲相的MIC分彆為:0.0313～0.2500、0.0625 ～ 1.0000、0.0313～1.0000、0.2500～4.0000、2.0000～32.0000 mg/L;對PM臨床人分離株酵母相的MIC分彆為:0.0039～0.2500、0.0313 ～ 0.5000、0.0313～ 2.0000、0.1250 ～ 2.0000、2.0000 ～ 16.0000mg/L.5種抗真菌藥物對廣西野生銀星竹鼠PM寄生株與臨床人PM分離株菌絲相和酵母相均敏感.同一溫度下伏立康唑對兩種不同來源PM的MIC最低,其他藥物的MIC依次為伊麯康唑、特比萘芬＜兩性黴素B＜氟康唑.同一藥物在同一溫度下對廣西野生銀星竹鼠PM寄生株與PM臨床分離株的MIC無明顯差異;伊麯康唑、兩性黴素B、特比萘芬對同一菌株在菌絲相和酵母相下的MIC存在差異.結論 PM對伏立康唑的敏感性最高;來自于廣西野生銀星竹鼠的PM寄生株與臨床人PM分離株對伏立康唑、伊麯康唑、特比萘芬、兩性黴素B及氟康唑的MIC類似;菌相的改變可影響PM對伊麯康唑、兩性黴素B、特比萘芬的敏感性.
목적 관찰마이니비청매(PM)엄서야생은성죽서기생주여림상인분리주대복립강서화궤충상용항진균약물적민감성.방법 채용미국림상실험실표준위원회( CLSI )M27-A2화M38-A방안중적미량희석법,측정복립강서、이곡강서、특비내분、량성매소B급불강서대14주엄서야생은성죽서PM기생주여25주림상인PM분리주25℃균사상급37 ℃효모상적최소억균농도(MIC).용량양본균수비교t검험비교PM기생주여림상인PM분리주MIC적차이성,용배대t검험비교동일주균재량충불동온도상하적MIC차이성.결과 복립강서、이곡강서、특비내분、량성매소B、불강서대PM기생주균사상적MIC분별위:0.0313 ～ 0.1250、0.1250 ～ 1.0000、0.0313 ～ 0.5000、0.2500 ～ 4.0000、2.0000 ～ 8.0000 mg/L;대PM기생주효모상적MIC분별위:0.0078 ～ 0.2500、0.0313～ 0.5000、0.0313 ～ 1.0000、0.2500～2.0000、1.0000～8.0000 mg/L;대PM림상인분리주균사상적MIC분별위:0.0313～0.2500、0.0625 ～ 1.0000、0.0313～1.0000、0.2500～4.0000、2.0000～32.0000 mg/L;대PM림상인분리주효모상적MIC분별위:0.0039～0.2500、0.0313 ～ 0.5000、0.0313～ 2.0000、0.1250 ～ 2.0000、2.0000 ～ 16.0000mg/L.5충항진균약물대엄서야생은성죽서PM기생주여림상인PM분리주균사상화효모상균민감.동일온도하복립강서대량충불동래원PM적MIC최저,기타약물적MIC의차위이곡강서、특비내분＜량성매소B＜불강서.동일약물재동일온도하대엄서야생은성죽서PM기생주여PM림상분리주적MIC무명현차이;이곡강서、량성매소B、특비내분대동일균주재균사상화효모상하적MIC존재차이.결론 PM대복립강서적민감성최고;래자우엄서야생은성죽서적PM기생주여림상인PM분리주대복립강서、이곡강서、특비내분、량성매소B급불강서적MIC유사;균상적개변가영향PM대이곡강서、량성매소B、특비내분적민감성.
Objective To test the susceptibility of Penicilliosis marneffei (PM) isolates from Guangxi bamboo rats and patients to voriconazole and several commonly used antifungal agents.Methods According to the Clinical and Laboratory Standards Institute (CLSI) M27-A2 and M38-A document,a microdilution method was used to determine the minimum inhibitory concentration (MIC) of voriconazole,itraconazole,terbinafine,amphotericin B,and fluconazole against mycelial phase (25 ℃) and yeast phase (37 ℃) of 14 PM isolates from Guangxi Bamboo rats and 25 PM isolates from patients.The difference in MIC of the antifungals was assessed by two-sample t test between Bamboo rat PM isolates and clinical PM isolates,and by paired t test between the mycelial and yeast phase of PM isolates.Results The MIC ranges of voriconazole,itraconazole,terbinafine,amphoteriein B and fluconazole were 0.0313-0.1250,0.1250-1.0000,0.0313-0.5000,0.2500-4.0000,2.0000-8.0000 mg/L,respectively for mycelial phase of Bamboo rat PM isolates,0.0078-0.2500,0.0313-0.5000,0.0313-1.0000,0.2500-2.0000,1.0000-8.0000 mg/L,respectively for yeast phase of Bamboo rat PM isolates,0.0313-0.2500,0.0625-1.0000,0.0313-1.0000,0.2500-4.0000,2.0000-32.0000 mg/L,respectively for mycelial phase of clinical PM isolates,0.0039-0.2500,0.0313-0.5000,0.0313-2.0000,0.1250-2.0000,2.0000-16.0000 mg/L,respectively for yeast phase of clinical PM isolates.None of the PM isolates was resistant to any of the antifungals.The MIC of voriconazole was found to be the lowest for PM isolates from both Bamboo rats and patients at the same temperature (37 ℃ or 25 ℃),followed by itraconazole,terbinafine,amphotericin B and fluconazole.Statistical difference was found in the MIC values of itraconazole,terbinafine,amphotericin B between the yeast and mycelial phase of the same PM isolate,but not found in antifungal MIC values between Bamboo rat isolates and clinical isolates at the same phase.Conclusions Of the tested drugs,voriconazole shows the strongest antifungal potency. The PM isolates from Guangxi Bamboo rats are similar to clinical PM isolates in the sensitivity to voriconazole,itraconazole,terbinafine,amphotericin B and fluconazole.The phase of PM isolates may affect their susceptibility to itraconazole,amphotericin B and terbinafine.
