安徽医药
安徽醫藥
안휘의약
ANHUI MEDICAL AND PHARMACEUTICAL JOURNAL
2009年
7期
749-750
,共2页
崔颖%戴萍萍%陆忠祥%路小东%吕凌
崔穎%戴萍萍%陸忠祥%路小東%呂凌
최영%대평평%륙충상%로소동%려릉
天麻素%再造浓缩丸%HPLC
天痳素%再造濃縮汍%HPLC
천마소%재조농축환%HPLC
gastrodin%zaizao pills%HPLC
目的 用反相高效液相色谱法测定再造浓缩丸中天麻素的含量.方法 用乙醚萃取法去除样品中的干扰物质.采用Cromosil C18分析柱(4.6 mm×250 mm,5 μm),流动相:乙腈-0.05%磷酸溶液(1.5∶ 98.5),检测波长:220 nm,流速:1 ml·min-1.结果 天麻素进样量在0.08～1.20 μg(r=0.999 9)范围内呈线性,加样回收率为98.77%(RSD=5.0%).结论 前处理方法 能有效去除干扰物质,保护色谱柱,所得样品组分分离度好,简便快速,结果 准确可靠,可用于制备再造浓缩丸质量控制所需的供试品溶液.
目的 用反相高效液相色譜法測定再造濃縮汍中天痳素的含量.方法 用乙醚萃取法去除樣品中的榦擾物質.採用Cromosil C18分析柱(4.6 mm×250 mm,5 μm),流動相:乙腈-0.05%燐痠溶液(1.5∶ 98.5),檢測波長:220 nm,流速:1 ml·min-1.結果 天痳素進樣量在0.08～1.20 μg(r=0.999 9)範圍內呈線性,加樣迴收率為98.77%(RSD=5.0%).結論 前處理方法 能有效去除榦擾物質,保護色譜柱,所得樣品組分分離度好,簡便快速,結果 準確可靠,可用于製備再造濃縮汍質量控製所需的供試品溶液.
목적 용반상고효액상색보법측정재조농축환중천마소적함량.방법 용을미췌취법거제양품중적간우물질.채용Cromosil C18분석주(4.6 mm×250 mm,5 μm),류동상:을정-0.05%린산용액(1.5∶ 98.5),검측파장:220 nm,류속:1 ml·min-1.결과 천마소진양량재0.08～1.20 μg(r=0.999 9)범위내정선성,가양회수솔위98.77%(RSD=5.0%).결론 전처리방법 능유효거제간우물질,보호색보주,소득양품조분분리도호,간편쾌속,결과 준학가고,가용우제비재조농축환질량공제소수적공시품용액.
Aim To determine gastrodin in Zaizao Nongsuo pills by RP-HPLC.Method Other substances in the pills were removed by ether extraction.The chromatographic method was carried out on Cromosil C18 column using acetonitrile- 0.05%phosphoric acid(1.5∶ 98.5)as a mobile phase.The detection wavelength was set at 220nm.The flow rate was 1 ml·min-1.Results The calibration curve shows a good linear correlation within the concentration range of 0.08～1.20 μg(r=0.999 9),and the average recovery was 98.77% with RSD of 5.0% for gastrodin.Conclusion The method can remove the other substances to protect the column.It is convenient,rapid,accurate and reliable,which can be applied to preparing the sample solution of zaizao nongsuo pills.
