CAJ | 학술논문

目的寻找肝纤维化最佳模型.方法将Wistar大鼠随机分成血清组、四氯化碳皮下注射组、四氯化碳腹腔注射组,每组30只,各组分别给予猪血清腹腔注射、40%四氯化碳皮下和腹腔注射造模(每周2次),观察造模过程中大鼠死亡情况以及4周及6周各组大鼠肝纤维化的程度.结果 3种方法都能成功制备肝纤维化模型.从动物死亡情况来看,四氯化碳腹腔注射组死亡率明显高于前两组;血清组死亡率最低,但与四氯化碳皮下注射组比较无显著差异;从模型形成时间来看,血清组造模时间较长,明显高于其他两组,四氯化碳皮下注射组与四氯化碳腹腔注射组在模型形成时间上无明显差异.结论四氯化碳皮下注射组制备肝纤维化模型动物死亡率较低,肝纤维化形成时间较短,是一种制作肝纤维化模型较好的方法.
목적 심조간섬유화최가모형.방법 장Wistar대서수궤분성혈청조、사록화탄피하주사조、사록화탄복강주사조,매조30지,각조분별급여저혈청복강주사、40%사록화탄피하화복강주사조모(매주2차),관찰조모과정중대서사망정황이급4주급6주각조대서간섬유화적정도.결과 3충방법도능성공제비간섬유화모형.종동물사망정황래간,사록화탄복강주사조사망솔명현고우전량조;혈청조사망솔최저,단여사록화탄피하주사조비교무현저차이;종모형형성시간래간,혈청조조모시간교장,명현고우기타량조,사록화탄피하주사조여사록화탄복강주사조재모형형성시간상무명현차이.결론 사록화탄피하주사조제비간섬유화모형동물사망솔교저,간섬유화형성시간교단,시일충제작간섬유화모형교호적방법.
Objective To find the best model of hepatic fibrosis. Methods Wistar rats were randomly allocated into three groups: pig serum group (n=30), subcutaneous injection carbon tetrachloride (CCl_4) group (n=30) and intraperitoneal injection CCl_4 group (n=30). Hepatic fibrosis was induced by subcutaneous, intraperitoneal injection of 40% CCl_4 or pig serum (two times per a week) for 4 weeks and 6 weeks respectively in rats. Rat mortality, change of liver tissue and degree of hepatic fibrosis in all groups were examined. Results Hepatic fibrosis models were induced successfully by the three different methods, but rat mortalities in the pig serum group and 40% CCl_4 subcutaneous injection group were significantly lower than in 40% CCl_4 intraperitoneal injection group, and the time of hepatic fibrosis model formation in the 40% CCl_4 group was shorter obviously than that in the other two groups. Conclusion Hepatic fibrosis induced by subcutaneous injection CCl_4 is the best model of hepatic fibrosis.