CAJ | 학술논문

目的:通过测定小鼠细胞免疫与体液免疫,来研究富硒大麦苗对小鼠免疫功能的调节作用.方法:用不同剂量富硒大麦苗(0.1、0.5、1g/ml)对小鼠进行灌胃,同时设正常大麦苗组(1g/ml)、生理盐水组对照组,各组灌胃量为每天0.2ml.测小鼠的白细胞总数、小鼠腹腔巨噬细胞吞噬功能、抗体效价、抗体生成细胞数量以及免疫器官重量等指标,并对其进行统计分析.结果:富硒大麦苗组与生理盐水组比较能显著提高小鼠腹腔巨噬细胞的吞噬能力(P<0.01),能明显提高抗鸡红细胞抗体的效价(P<0.05),明显提高脾脏指数(P<0.05),明显提高小鼠白细胞总数(P<0.05).结论:富硒大麦茁有显著提高小鼠细胞免疫与体液免疫的功能,并与其剂量呈正相关.
목적:통과측정소서세포면역여체액면역,래연구부서대맥묘대소서면역공능적조절작용.방법:용불동제량부서대맥묘(0.1、0.5、1g/ml)대소서진행관위,동시설정상대맥묘조(1g/ml)、생리염수조대조조,각조관위량위매천0.2ml.측소서적백세포총수、소서복강거서세포탄서공능、항체효개、항체생성세포수량이급면역기관중량등지표,병대기진행통계분석.결과:부서대맥묘조여생리염수조비교능현저제고소서복강거서세포적탄서능력(P<0.01),능명현제고항계홍세포항체적효개(P<0.05),명현제고비장지수(P<0.05),명현제고소서백세포총수(P<0.05).결론:부서대맥촬유현저제고소서세포면역여체액면역적공능,병여기제량정정상관.
Objective: To observe immune regulation function of selenium-enriched barley seedling (SEBS) in mice by detecting cell-mediated immunity and humoral immunity. Methods: Experimental mice were orally administered with SEBS at doses of 1,0.5 g/ml and 0.1 g/ml. Meanwhile, two groups were also orally administered with saline water at the same volume and regular barley seedling at the dose of 1 g/ml as the controls. Total number of white blood cell (WBC), phagocytosis test of macrophage on abdominal cavity, titer of antibody, number of antibody-producing cells and weight of immune organs in mice were determined. Results: Compared to saline water group, SEBS could significantly improve phagocytic function of macrophages (P < 0.01), obviously increase the titer of anti-CRBC antibody (P < 0.05), spleen index (P < 0.05), and total number of WBC (P<0.05). Conclusion: SEBS can promote cell-mediated immunity and humoral immunity in mice in a dose-dependent manner.