西北植物学报
西北植物學報
서북식물학보
ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA
2010年
1期
8-13
,共6页
盾叶薯蓣%环阿屯醇合酶%薯蓣皂甙元生物合成%基因克隆
盾葉藷蕷%環阿屯醇閤酶%藷蕷皂甙元生物閤成%基因剋隆
순협서여%배아둔순합매%서여조대원생물합성%기인극륭
Dioscorea zingiberensis Wright%cycloartenol synthase%diosgenin biosynthesis%gene cloning
环阿屯醇合酶(cycloartenol synthase,CAS)是薯蓣皂甙元生物合成途径中的第一个关键酶.以基因组DNA为模板,利用染色体步行和长距离PCR方法首次克隆了盾叶薯蓣CAS全长基因.序列分析比较结果表明,盾叶薯蓣CAS全长基因为7 192 bp,由18个外显子和17个内含子组成.外显子总长为2 280 bp,编码759个氨基酸,最长的外显子为198 bp,最短的为47 bp;内含子总长4 912 bp,最长的内含子为1 551 bp,最短的为68 bp.Southern blot杂交分析表明,CAS基因在盾叶薯蓣基因组中为单拷贝.
環阿屯醇閤酶(cycloartenol synthase,CAS)是藷蕷皂甙元生物閤成途徑中的第一箇關鍵酶.以基因組DNA為模闆,利用染色體步行和長距離PCR方法首次剋隆瞭盾葉藷蕷CAS全長基因.序列分析比較結果錶明,盾葉藷蕷CAS全長基因為7 192 bp,由18箇外顯子和17箇內含子組成.外顯子總長為2 280 bp,編碼759箇氨基痠,最長的外顯子為198 bp,最短的為47 bp;內含子總長4 912 bp,最長的內含子為1 551 bp,最短的為68 bp.Southern blot雜交分析錶明,CAS基因在盾葉藷蕷基因組中為單拷貝.
배아둔순합매(cycloartenol synthase,CAS)시서여조대원생물합성도경중적제일개관건매.이기인조DNA위모판,이용염색체보행화장거리PCR방법수차극륭료순협서여CAS전장기인.서렬분석비교결과표명,순협서여CAS전장기인위7 192 bp,유18개외현자화17개내함자조성.외현자총장위2 280 bp,편마759개안기산,최장적외현자위198 bp,최단적위47 bp;내함자총장4 912 bp,최장적내함자위1 551 bp,최단적위68 bp.Southern blot잡교분석표명,CAS기인재순협서여기인조중위단고패.
Dioscorea zingiberensis Wright is the main material in China for production of diosgenin.Cycloartenol synthase (CAS) is the first committed enzyme for diosgenin biosynthesis.The full-length CAS gene was cloned from D.zingiberensis for the first time by Chromosome Walking and Long-distance PCR methods using the genomic DNA as templates.Sequence analysis showed that the full-length CAS gene of D.zingiberensis was 7 192 bp,consisting of 18 exons and 17 introns.Total length of exons was 2 280 bp,encoding 759 amino acids;The longest exon was 198 bp and the shortest one was 47 bp;Total length of introns was 4 912 bp,the longest intron was 1 551 bp and the shortest one was 68 bp.Southern blot analysis revealed that there was a single copy of CAS gene in D.zingiberensis genome.
