CAJ | 학술논문

目的研究N-甲基亚硝基脲诱导大鼠原位膀胱癌模型的构建过程以及计算机断层扫描对大鼠原位膀胱癌的诊断价值.方法 50只SD大鼠随机分为两组,对照组A组15只,实验组B组35只.B组大鼠经尿道膀胱内灌注MNU每两周一次,每次2 mg,共4次.A组大鼠用生理盐水以同样的方法灌注.于第14周末对两组大鼠进行膀胱CT扫描诊断,并且处死后取膀胱组织行病理学观察.结果 B组28只大鼠CT扫描膀胱均见明显异常,表现为局部肿块突起或膀胱壁不规则增厚、密度不均,病理诊断均为膀胱癌.A组13只大鼠经CT扫描未发现膀胱肿瘤,病理检查未见肿瘤组织.结论 MNU可以诱导大鼠原位膀胱癌模型的建立.CT扫描可作为大鼠原位膀胱癌诊断的可靠方法,并能为肿瘤的化疗或放疗等后续实验提供更好的大鼠模型.
목적 연구N-갑기아초기뇨유도대서원위방광암모형적구건과정이급계산궤단층소묘대대서원위방광암적진단개치.방법 50지SD대서수궤분위량조,대조조A조15지,실험조B조35지.B조대서경뇨도방광내관주MNU매량주일차,매차2 mg,공4차.A조대서용생리염수이동양적방법관주.우제14주말대량조대서진행방광CT소묘진단,병차처사후취방광조직행병이학관찰.결과 B조28지대서CT소묘방광균견명현이상,표현위국부종괴돌기혹방광벽불규칙증후、밀도불균,병리진단균위방광암.A조13지대서경CT소묘미발현방광종류,병리검사미견종류조직.결론 MNU가이유도대서원위방광암모형적건립.CT소묘가작위대서원위방광암진단적가고방법,병능위종류적화료혹방료등후속실험제공경호적대서모형.
Objective To establish an orthotopic rat model of bladder cancer induced by N-methyl.nitrosourea (MNU)and to evaluate the diagnostic value of CT(computed tomography)scanning in this experimental model.Methods 50 SD rats were randomly divided into the control group(group A)with 15 rats and experimental group(group B)with 35 rats.Each rat of group B was treated with 2 mg MNU per dose every other week,totally 4 doses,by per urethra administration.Meanwhile,each rat of group A was treated with normal saline.Then,at the 14th week,all the rats were evaluated by CT scanning and pathological examination.Results Abnormal changes were detected in each of the 28 rats in group B by CT scanning,and manifested as local mass,thickening bladder wall accompanied with heterogeneous density.Bladder cancer was diagnosed by pathology.However,no bladder tumor was detected by CT scanning and pathologicalexamination in group A.Conclusion A rat model of orthotopic bladder cancer can be established by per urethra administration of MNU.CT scanning is a reliable diagnostic technique concerning this model.Furthermore,this technique can render US a more suitable rat model for further experimental studies on chemotherapy and radiotherapy of bladder Cancer.