CAJ | 학술논문

目的研究天芪降糖胶囊对糖耐量减低患者血清代谢物的影响,探讨天芪降糖胶囊的作用途径.方法应用UPLC/TOF-MS液质联用分析方法,建立人血清代谢指纹谱,进行主成分分析(PCA)和偏最小二乘法一判别分析(PLS-DA),观察天芪降糖胶囊的干预效果.结果治疗3个月、6个月后受试者血清代谢物发生了明显变化,天芪降糖胶囊参与机体在磷脂、糖脂、核苷、肉碱等代谢水平的修复过程.结论应用代谢组学方法,可以从整体出发研究天芪降糖胶囊对机体的作用,从小分子代谢物变化衡量其疗效.
목적 연구천기강당효낭대당내량감저환자혈청대사물적영향,탐토천기강당효낭적작용도경.방법 응용UPLC/TOF-MS액질련용분석방법,건립인혈청대사지문보,진행주성분분석(PCA)화편최소이승법일판별분석(PLS-DA),관찰천기강당효낭적간예효과.결과 치료3개월、6개월후수시자혈청대사물발생료명현변화,천기강당효낭삼여궤체재린지、당지、핵감、육감등대사수평적수복과정.결론 응용대사조학방법,가이종정체출발연구천기강당효낭대궤체적작용,종소분자대사물변화형량기료효.
Objective To study the effects of tiangijiangtang capsule on lipid metabolomics in impaired glucose tolerance patients. MethodsA UPLC/TOF-MS based methods were used for serum metabolic fingerprinting analysis. Multivariate statistical analysis was used to analyze the metabonome difference. ResultsThe statistically significant changes were found in levels of lipids, acylcarnitine, glycosphingolipid and glucuronidation (all P＜0.05) after the treatment with tianqijiangtang capules for 3 and 6 months as compared with untreated people. Conclusion The UPLC/TOF-MS based metabonomic approach can give some explain to the pathophysiology of impaired glucose tolerance, as a supplement for clinical diagnosis and therapy.