CAJ | 학술논문

目的初步探讨L-精氨酸诱导慢性胰腺炎大鼠动物模型的可行性.方法将实验动物按完全随机法分为对照组及精氨酸12、24 h和7 d组,每组10只,间隔1 h分两次腹腔内注射L-精氨酸溶液造模.造模后相应时间点检测血淀粉酶、血糖水平,对胰腺组织进行病理评分,Van Gieson法对胰腺胶原纤维染色.结果对照组及精氨酸12、24 h和7 d组的血淀粉酶水平分别为(1 634±890)U/L、(3 872±2 676)U/L、(3 307±2 197)U/L和(1 561±304)U/L,精氨酸7 d组血淀粉酶水平显著低于12 h和24 h组(P＜0.05),恢复到对照组水平.各组间血糖水平无明显差异.对照组及精氨酸12、24 h和7 d组胰腺的病理分值分别为0.8±0.4、5.1±2.6、6.5±2.2和4.5±1.6,精氨酸7 d组胰腺病理评分显著低于24 h组(P＜0.05),但仍显著高于对照组(P＜0.05).精氨酸7 d组胶原染色范围明显增加,其他各组未见明显胶原染色.结论 L-精氨酸腹腔注射后7 d可引起胰腺组织纤维化及管状复合结构增生,可用于探索慢性胰腺炎大鼠模型.
목적 초보탐토L-정안산유도만성이선염대서동물모형적가행성.방법 장실험동물안완전수궤법분위대조조급정안산12、24 h화7 d조,매조10지,간격1 h분량차복강내주사L-정안산용액조모.조모후상응시간점검측혈정분매、혈당수평,대이선조직진행병리평분,Van Gieson법대이선효원섬유염색.결과 대조조급정안산12、24 h화7 d조적혈정분매수평분별위(1 634±890)U/L、(3 872±2 676)U/L、(3 307±2 197)U/L화(1 561±304)U/L,정안산7 d조혈정분매수평현저저우12 h화24 h조(P＜0.05),회복도대조조수평.각조간혈당수평무명현차이.대조조급정안산12、24 h화7 d조이선적병리분치분별위0.8±0.4、5.1±2.6、6.5±2.2화4.5±1.6,정안산7 d조이선병리평분현저저우24 h조(P＜0.05),단잉현저고우대조조(P＜0.05).정안산7 d조효원염색범위명현증가,기타각조미견명현효원염색.결론 L-정안산복강주사후7 d가인기이선조직섬유화급관상복합결구증생,가용우탐색만성이선염대서모형.
Objective To investigate the feasibility of induction of experimental chronic pancreatitis rat model with L-arginine.Methods Animals were randomly divided into control group,arginine 12 h group,arginine 24 h group and arginine 7 d group with 10 rats in each group.L-arginine solution was intraperitoneally injected twice with an interval of 1 h.Serum amylase and glucose levels at corresponding time points were detected and histopathological scores of pancreas were evaluated.Collagen in pancreas was stained with Van Gieson method.Results Serum amylase levels were (1 634±890 ) U/L,( 3 872±2 676 ) U/L,( 3 307±2 197)U/L and (1 561±304) U/L in control group,arginine 12 h group,arglnine 24 h group and arginine 7 d group,respectively.The serum amylase level in arginine 7 d group was significantly lower than those in arginine 12 h group and arginine 24 h group (P ＜ 0.05 ).There was no significant difference in serum glucose level among all the groups.Histopathological scores were 0.8±0.4,5.1±2.6,6.5±2.2 and 4.5±1.6,respectively.The histopathological score of arginine 7 d group was significantly lower than those in arginine 24 h group (P ＜ 0.05 ).Obvious collagen could be found in pancreatic parenchyma in arginine 7 d group,while little collagen was found in pancreatic tissue in control,arginine 12 h and arginine 24 h groups.Conclusions Injection of L-arglnine induced fibrosis in pancreatic parenchyma and proliferation of tubular complex 7 days later,and it could be used for chronic pancreatitis model induction.