云南省一株版纳病毒的分离和鉴定
운남성일주판납병독적분리화감정
Isolation and identification of a Banna virus strain from mosquitoes in Yunnan province
  • 万方数据
    中华流行病学杂志 중화류행병학잡지
    CHINESE JOURNAL OF EPIDEMIOLOGY
    2010年 6期 685-687 ,共3页

    孙肖红%张晓龙%刘宇夫%王静%魏莲%高旭%赵文%张勋%杨学兵%张乐

    손초홍%장효룡%류우부%왕정%위련%고욱%조문%장훈%양학병%장악

    版纳病毒%序列分析 版納病毒%序列分析
    판납병독%서렬분석
    Banna virus%Sequence analysis
    目的 对2008年在云南省勐腊县采集的蚊虫进行版纳病毒(BAV)分离和鉴定.方法 2008年7月在勐腊县南嘎村人房和畜圈采集蚊虫标本,用细胞培养法进行虫媒病毒分离并鉴定,测定分离到的BAV第5、8及11节段序列,利用MEGA4软件进行系统进化分析.结果 共采集到蚊虫7种1731只,分离到1株导致C6/36细胞产生规律病变的分离物,经血清学和分子生物学鉴定为BAV,系统进化分析显示第8节段和中国分离株关系最近,而第11节段和越南分离株关系最近.结论 系统进化分析提示该BAV毒株可能与越南分离株之间发生了基因重配.
    목적 대2008년재운남성맹석현채집적문충진행판납병독(BAV)분리화감정.방법 2008년7월재맹석현남알촌인방화축권채집문충표본,용세포배양법진행충매병독분리병감정,측정분리도적BAV제5、8급11절단서렬,이용MEGA4연건진행계통진화분석.결과 공채집도문충7충1731지,분리도1주도치C6/36세포산생규률병변적분리물,경혈청학화분자생물학감정위BAV,계통진화분석현시제8절단화중국분리주관계최근,이제11절단화월남분리주관계최근.결론 계통진화분석제시해BAV독주가능여월남분리주지간발생료기인중배.
    Objective To isolate and identify Banna virus(BAV)from mosquitoes collected in Mengla county of Yunnan province.Methods Mosquito samples were collected in houses and stock yards in Mengla county,2008.Mosquitoes were homogenized and incubated onto both C6/36 and BHK21 cells.The new isolate was identified by using ELISA and RT-PCR.The sequences of segment 5.8 and 11 of BAV were amplified by RT-PCR and determined.Phylogenetic analysis on the new BAV were performed using MEGA4 program.Results 1731 mosquitoes representing 7 species were collected with one strain of BAV isolated and identified.Phylogenetic analysis based on sequences of segment 8 showed the new isolate was closed to BAV strain isolated in Yunnan,but segment 11 sequence was closed to Vietnam strain.Conclusion Results of phylogenetic analysis implied that the BAV re-assortment might have been occurred both in Chinese and Vietnam strains.
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