CAJ | 학술논문

目的通过分析外周血T淋巴细胞CD8分子表达水平,了解职业性慢性铅中毒患者异常的免疫功能状态.方法对23例职业性慢性铅中毒患者(铅中毒组)及20例健康非职业铅接触成人(对照组)采用流式细胞技术检测外周血T淋巴细胞CD8分子表达水平,统计CD8低表达(CD8low)细胞群和正常表达(CD8nomal)细胞群的数量.结果与对照组(8.21%±3.02%)比较,铅中毒组CD8low细胞群相对百分比(12.98%±5.62%)明显增加,差异有统计学意义(P＜0.05);两组CD8nomal细胞群数量则无明显变化.结论职业性慢性铅中毒患者外周血CD8+T淋巴细胞虽然总数没有异常,但CD8分子表达出现了弱化.该现象可能是铅致免疫功能损伤重要表现之一,CD8low细胞群有望成为研究铅免疫毒性新的线索.
목적 통과분석외주혈T림파세포CD8분자표체수평,료해직업성만성연중독환자이상적면역공능상태.방법 대23례직업성만성연중독환자(연중독조)급20례건강비직업연접촉성인(대조조)채용류식세포기술검측외주혈T림파세포CD8분자표체수평,통계CD8저표체(CD8low)세포군화정상표체(CD8nomal)세포군적수량.결과 여대조조(8.21%±3.02%)비교,연중독조CD8low세포군상대백분비(12.98%±5.62%)명현증가,차이유통계학의의(P＜0.05);량조CD8nomal세포군수량칙무명현변화.결론 직업성만성연중독환자외주혈CD8+T림파세포수연총수몰유이상,단CD8분자표체출현료약화.해현상가능시연치면역공능손상중요표현지일,CD8low세포군유망성위연구연면역독성신적선색.
Objective To analyze the changes in CD8low T lymphocyte subsets in patients with occupational chronic lead poisoning. Methods Flow cytometric analysis was used to count the numbers of CD8+ cells. 23 patients with occupational chronic lead poisoning and 20 controls were examined. Results Compared with control group (8.21%±3.02%), the CD8low T lymphocyte (12.98%±5.62%) were significantly increased in patients with occupational chronic lead poisoning. Conclusion Although the ratio of CD +T lymphocyte is normal, the CD8 level is significantly decreased. The increase of CD8low T lymphocyte may be an important phenomenon of immuno-injury induced by lead. CD8low T lymphocyte could be an new direction for research of lead immuno-toxicity.