CAJ | 학술논문

目的通过密码子优化提高重组腺病毒中人轮状病毒VP6基因的表达量.方法人工合成按照人的优势密码子优化的人轮状病毒VP6基因,包装重组腺病毒后,与未优化的相同基因的重组腺病毒用免疫荧光和Western Blot方法比较其表达量.结果密码子优化的人轮状病毒VP6基因,与未优化的相同基因的重组腺病毒比较,其蛋白表达量大大提高.结论密码子优化确实提高了重组腺病毒中人轮状病毒VP6基因的表达量,可望满足腺病毒载体疫苗的研发需要.
목적 통과밀마자우화제고중조선병독중인륜상병독VP6기인적표체량.방법 인공합성안조인적우세밀마자우화적인륜상병독VP6기인,포장중조선병독후,여미우화적상동기인적중조선병독용면역형광화Western Blot방법비교기표체량.결과 밀마자우화적인륜상병독VP6기인,여미우화적상동기인적중조선병독비교,기단백표체량대대제고.결론 밀마자우화학실제고료중조선병독중인륜상병독VP6기인적표체량,가망만족선병독재체역묘적연발수요.
Objective To increase the recombinant adenovirus vector mediated human rotavirus VP6 gene expression through coden optimization.Methods We have artificially synthesized VP6 gene of group A human rotavirus according to the human biased codon.The modified gene was transfected into 293 cells using adenovirus vector and the gene product,the respective protein was produced.The expression level of optimized gene and wild type gene was detected by Immunofluorescence(IF) and Western Blot.Result A remarkable increase of the expression level of optimized VP6 gene in comparison with the wild-type control.Conclusion The coden optimization indeed help increasing the recombinant adenovirus mediated human rotavirus gene expression,which indicated the potential application of such recombinant adenoviruses in the development of adenoviral-vectored rotavirus vaccines.