CAJ | 학술논문

目的了解含有EB病毒潜伏膜蛋白2的非复制型重组腺病毒(Ad5F35-LMP2)修饰的DC能否在体内抑制肿瘤细胞生长.方法人外周血单个核细胞在细胞因子IL-4、GM-CSF和TNF-a诱导下生成树突状细胞.将人的外周血单个核细胞移植入实验组SCID小鼠体内2次,再分别经两次DC与Ad5F35-LMP2-DC免疫SCID小鼠,然后用1×106的CNE-2肿瘤细胞接种SCID小鼠皮下,观察肿瘤生长情况.结果 Ad5F35-LMP2-DC组肿瘤的潜伏期明显延长,对照组和DC组小鼠1周左右就出现了肿瘤,而Ad5F35-LMP2-DC组则直到2周左右才出现肿瘤;且肿瘤体积最小,重量最轻,与DC组比Ad5F35-LMP2-DC疫苗组的肿瘤抑制率为64.75%.结论 Ad5F35-LMP2重组腺病毒疫苗修饰的DC在体内可以有效的抑制NPC肿瘤细胞CNE-2生长.
목적 료해함유EB병독잠복막단백2적비복제형중조선병독(Ad5F35-LMP2)수식적DC능부재체내억제종류세포생장.방법 인외주혈단개핵세포재세포인자IL-4、GM-CSF화TNF-a유도하생성수돌상세포.장인적외주혈단개핵세포이식입실험조SCID소서체내2차,재분별경량차DC여Ad5F35-LMP2-DC면역SCID소서,연후용1×106적CNE-2종류세포접충SCID소서피하,관찰종류생장정황.결과 Ad5F35-LMP2-DC조종류적잠복기명현연장,대조조화DC조소서1주좌우취출현료종류,이Ad5F35-LMP2-DC조칙직도2주좌우재출현종류;차종류체적최소,중량최경,여DC조비Ad5F35-LMP2-DC역묘조적종류억제솔위64.75%.결론 Ad5F35-LMP2중조선병독역묘수식적DC재체내가이유효적억제NPC종류세포CNE-2생장.
Objective To observe whether dendritic cells(DCs) transfected with recombinant adenovirus Ad5F35-LMP2 suppress the proliferation of CNE-2 in vivo. Methods Dendritic cells have been generated with the induction of IL-4,GM-CSF and TNF-a in vitro. After human PBMCs were transplant into SCID mice,SCID mice were vaccinated with Ad5F35-LMP2-DC or untransfected DC. And four days latter,CNE-2 (1×106cell for each mouse) inoculated into SCID mouse subcutaneously. The tumor growth was monitored every week. Results The tumor latent period of Ad5F35-LMP2-DC group is longer among three groups. Untransfected DC group and CNE-2 positive control group could be found tumor about one week, but Ad5F35-LMP2-DC group was about two weeks. And the tumor was smaller and lighter than untransfected DC group and CNE-2 positive control group. Average tumor control rate reached 64.75% compared with untransfected DC group. Conclusion Ad5F35-LMP2-DC could effectively suppress the proliferation of CNE2 in vivo.