CAJ | 학술논문

[目的]检测猪CACNA2D1基因第14内含子的多态性,为猪肉质性状的分子标记辅助选择提供条件.[方法]采用PCR-SSCP检测猪CACNA2D1基因第14外显子和内含子在6个猪群中的DNA 多态性,并分析不同基因型对猪眼肌和腿臀肌肉pH45的遗传效应.[结果]猪CACNA2D1基因第14内含子有3 种基因型,即AA、AG 和GG,在其序列(GenBank接受号:FJ156361)的1 145位碱基存在1个多态位点(G→A).χ2 独立性检验结果表明,这3种基因型在各猪群间的分布差异显著(P<0.05).AA基因型金华×皮特兰F2资源家系猪与AG基因型猪在眼肌和腿臀肌肉pH45值上的差异显著(P<0.05).[结论]猪CACNA2D1基因第14内含子的多态性影响猪眼肌和腿臀肌pH45值.
[목적]검측저CACNA2D1기인제14내함자적다태성,위저육질성상적분자표기보조선택제공조건.[방법]채용PCR-SSCP검측저CACNA2D1기인제14외현자화내함자재6개저군중적DNA 다태성,병분석불동기인형대저안기화퇴둔기육pH45적유전효응.[결과]저CACNA2D1기인제14내함자유3 충기인형,즉AA、AG 화GG,재기서렬(GenBank접수호:FJ156361)적1 145위감기존재1개다태위점(G→A).χ2 독립성검험결과표명,저3충기인형재각저군간적분포차이현저(P<0.05).AA기인형금화×피특란F2자원가계저여AG기인형저재안기화퇴둔기육pH45치상적차이현저(P<0.05).[결론]저CACNA2D1기인제14내함자적다태성영향저안기화퇴둔기pH45치.
[Objective]To detect polymorphism of the14th intron of porcine CACNA2D1 gene, and thus provide conditions for the marker-assisted selection of pork quality traits. [Method] The polymorphism of the14th exon and intron of porcine CACNA2D1 gene was detected in six breeds by PCR-SSCP. The genetic effects of different genotypes on the pH45 value of eye muscle and ham joint muscle were also analyzed. [Result] There were three kinds of genotypes, namely, AA, AG and GG, in the 14th intron of porcine CACNA2D1 gene. The polymorphic locus (G → A) was at the 1 145 nt of the sequence (GenBank Accepted No. FJ156361). The results of χ2 test showed that the distribution of these three kinds of genotypes was significantly different in the six breeds (P＜0.05). The pH45 values of eye muscle and ham joint muscle were significantly different between genotype AA and genotype AG of F2 generation from the Jianhua×Pietrain resource family (P＜0.05). [Conclusion] The polymorphism of the4th intron of porcine CACNA2D1 gene has effects on the pH45 values of eye muscle and ham joint muscle.