CAJ | 학술논문

本试验以萝卜品种"鲁萝卜一号"为试材,对其不定芽和愈伤组织的诱导、增殖和分化进行了探讨.结果表明:带下胚轴的茎端为不定芽诱导适宜外植体;MS+3mg/L 6-BA+0.2 mg/L NAA为不定芽继代增殖的适宜培养基,但在该种培养基上继代5次后,需适当提高6-BA浓度,才能保持较高的增殖水平;以子叶为外植体,培养基为MS+3mg/L 6-BA+0.3mg/L NAA时,有利于愈伤组织的诱导;在此培养基中添加5mg/L AgNO3有利于愈伤组织继代与保存;愈伤组织转接在培养基MS+6mg/L 6-BA+0.1mg/L NAA+5mg/L AgNO3上,获得了再分化芽.
본시험이라복품충"로라복일호"위시재,대기불정아화유상조직적유도、증식화분화진행료탐토.결과표명:대하배축적경단위불정아유도괄의외식체;MS+3mg/L 6-BA+0.2 mg/L NAA위불정아계대증식적괄의배양기,단재해충배양기상계대5차후,수괄당제고6-BA농도,재능보지교고적증식수평;이자협위외식체,배양기위MS+3mg/L 6-BA+0.3mg/L NAA시,유리우유상조직적유도;재차배양기중첨가5mg/L AgNO3유리우유상조직계대여보존;유상조직전접재배양기MS+6mg/L 6-BA+0.1mg/L NAA+5mg/L AgNO3상,획득료재분화아.