CAJ | 학술논문

[目的]探索制备D-鸟氨酸盐酸盐的新方法.[方法] 采用L-鸟氨酸(L-Orn)为消旋反应原料,经消旋反应得到DL-Orn,然后利用蜂房哈夫尼菌AS1.1009中的赖氨酸脱羧酶进行DL-Orn酶法转化,可同时获得D-Orn和腐胺,并以此作为生物转化底物,考察了在水杨醛催化下,不同浓度NaOH水溶液对L-Orn消旋率和溶液中鸟氨酸总含量的影响.[结果] 通过化学酶法制备的D-鸟氨酸盐酸盐,收率为46.1%.确定在回流条件下使用1.0 mol/L氢氧化钠水溶液,用0.10摩尔比的水杨醛催化L-鸟氨酸在3 h内可生成DL-鸟氨酸.对生物转化中赖氨酸脱羧酶性质的研究表明,添加5 mmol/L的Fe2+ 可将比酶活提高为6 717 U.在此优化条件下,D-鸟氨酸可完全降解,最佳生物转化时间为16 h.[结论] 该研究为制备D-鸟氨酸提供了一种新的方法.
[목적]탐색제비D-조안산염산염적신방법.[방법] 채용L-조안산(L-Orn)위소선반응원료,경소선반응득도DL-Orn,연후이용봉방합부니균AS1.1009중적뢰안산탈최매진행DL-Orn매법전화,가동시획득D-Orn화부알,병이차작위생물전화저물,고찰료재수양철최화하,불동농도NaOH수용액대L-Orn소선솔화용액중조안산총함량적영향.[결과] 통과화학매법제비적D-조안산염산염,수솔위46.1%.학정재회류조건하사용1.0 mol/L경양화납수용액,용0.10마이비적수양철최화L-조안산재3 h내가생성DL-조안산.대생물전화중뢰안산탈최매성질적연구표명,첨가5 mmol/L적Fe2+ 가장비매활제고위6 717 U.재차우화조건하,D-조안산가완전강해,최가생물전화시간위16 h.[결론] 해연구위제비D-조안산제공료일충신적방법.
[Objective]The purpose was to explore the new method of preparing D-ornithine hydrochloride. [Method] With L-Ornithine (L-Orn) as the raw material for racemization reaction, DL-Orn was obtained through the racemization reaction, and then DL-Orn and putrescine was produced by means of biotransformation of DL-ornithine with lysine decarboxylase in Hafnia alvei AS1.1009 at same time, with which as the substrate of biotransformation, the effect of different concn. of NaOH water solution on DL-Orn rate and total amount of ornithine in the solution were investigated under salicylaldehyde catalysis. [Result] The D-ornithine hydrochloride prepared with chemoenzymatic method had yield of 46.1%. It was confirmed that in the water solution with 1.0 mol/L NaOH under return flow conditions, D-ornithine could be produced in the reaction process of racemizing L-ornithine by using 0.10 molar equivalent of salicylaldehyde within 3 h. The study on the character of lysine decarboxylase in biotransformation showed that adding 5 mmol/L Fe2+ ion could increase the specific activity up to 6 717 U. Under the optimization conditions, L-ornithine could be completely degraded by the decarboxylase, with the optimum time for biotransformation of 16 h. [Conclusion] This study peocided a new method for preparing D-ornithine.