CAJ | 학술논문

在前期获得11个转胆碱脱氢酶基因(betA)小黑杨株系的基础上,将转基因株系与非转基因对照株系在轻度盐碱地上造林,5 a后对试验林的转基因株系进行PCR分子检测,并运用SPSS软件对其树高、胸径、材积和保存率4个生长性状进行了方差分析.结果表明:转betA基因小黑杨外源基因稳定,目前尚未丢失;转基因株系与对照株系间树高、胸径、材积和保存率的差异均达到极显著水平;利用隶属函数法选择的转基因株系T1、T6和T8,为生长速度快、耐盐性高的优良株系.
재전기획득11개전담감탈경매기인(betA)소흑양주계적기출상,장전기인주계여비전기인대조주계재경도염감지상조림,5 a후대시험림적전기인주계진행PCR분자검측,병운용SPSS연건대기수고、흉경、재적화보존솔4개생장성상진행료방차분석.결과표명:전betA기인소흑양외원기인은정,목전상미주실;전기인주계여대조주계간수고、흉경、재적화보존솔적차이균체도겁현저수평;이용대속함수법선택적전기인주계T1、T6화T8,위생장속도쾌、내염성고적우량주계.
An afforestation test of transgenic and untransformed control plants was conducted on low-grade salinate fields by means of the obtained 11 betA transgenic Populus simonii × P. nigra plants in the previous work. Five years later, PCR detection for the transgenic plants was done, and variance analysis for tree height, diameter at breast height, volume, and survival rate was also made with SPSS software. Result showed that betA gene still existed in the genome of the transgenic plants. Significant variations were found in tree height, diameter at breast height, volume, and survival rate between the transgenic and untransformed control plants. Tl, T6 and T8 transgenic lines were identified as fast growing plants with high saline tolerance by subordinate function.