CAJ | 학술논문

目的探讨金黄色葡萄球菌分泌的趋化抑制蛋白(CHIPs)与G蛋白偶联受体(GPCR)C5a受体(C5aR)或促酰化蛋白受体(C512))的相互作用.方法将纯化的CHIPs蛋白分别与表达C5aR、C5L2、CXC趋化因子受体3(CXCR3)的人肾胚胎上皮细胞293T分别孵育,用Western blot检测结合情况.结果 CHIPs蛋白与C5aR相结合,与C5L2和CXCR3尤明显结合.结论 C5aR是CHIPs蛋白的受体,提示C5L2与C5aR在和CHIPs的结合作用上存在明显差异.
목적 탐토금황색포도구균분비적추화억제단백(CHIPs)여G단백우련수체(GPCR)C5a수체(C5aR)혹촉선화단백수체(C512))적상호작용.방법 장순화적CHIPs단백분별여표체C5aR、C5L2、CXC추화인자수체3(CXCR3)적인신배태상피세포293T분별부육,용Western blot검측결합정황.결과 CHIPs단백여C5aR상결합,여C5L2화CXCR3우명현결합.결론 C5aR시CHIPs단백적수체,제시C5L2여C5aR재화CHIPs적결합작용상존재명현차이.
Objective To investigate the mutual effect between G protein-coupled receptors(GPCR)including C5a receptor(C5aR),C5a like receptor 2(C5L2)and chemotaxis inhibitory protein(CHIPs)secreted by Staphylococcus aureus.Methods The purified CHIPs was incubated with HEK 293T cells overexpressing C5aR,C5L2 and C-X-C chemokine receptor type 3(CXCR3).Binding signal was detected by Western blot.Results HEK 293T cells overexpressing C5aR can efficiently bind to CHIPs.No apparent relation between CHIPs and C5L2 or CXCR3 was identified.Conclusion C5aR but not C5L2 is a membrane receptor for binding CHIPs,suggesting a difference between C5aR and C5L2 in association with binding CHIPs.