CAJ | 학술논문

以药用植物艾纳香为研究对象,以-20 ℃保存、4 ℃保存、室温自然干燥和硅胶干燥四种样品保存方式,并采用SDS法、CTAB法、SDS-CTAB法和改良CTAB法4种不同的基因组DNA提取方法进行了对比试验,以期建立艾纳香的较好的样品保存方法和基因组DNA提取方法.结果表明,-20 ℃保存是艾纳香的较理想的样品保存方式;改良CTAB法是艾纳香基因组DNA提取较适宜的方法,该方法提取的DNA经紫外检测,其A260/A280为1.8左右,明显优于SDS法(1.1～1.5)、CTAB法(1.2～1.5)和SDS-CTAB法(1.4～1 6),琼脂糖凝胶电泳、酶切检测和PCR扩增也得出了同样的结论.
이약용식물애납향위연구대상,이-20 ℃보존、4 ℃보존、실온자연간조화규효간조사충양품보존방식,병채용SDS법、CTAB법、SDS-CTAB법화개량CTAB법4충불동적기인조DNA제취방법진행료대비시험,이기건립애납향적교호적양품보존방법화기인조DNA제취방법.결과표명,-20 ℃보존시애납향적교이상적양품보존방식;개량CTAB법시애납향기인조DNA제취교괄의적방법,해방법제취적DNA경자외검측,기A260/A280위1.8좌우,명현우우SDS법(1.1～1.5)、CTAB법(1.2～1.5)화SDS-CTAB법(1.4～1 6),경지당응효전영、매절검측화PCR확증야득출료동양적결론.
The paper dealt with setting up a more suitable sample preservation way and genomic DNA extraction method for Blumea balsamifera by comparing and analysing four sample preservation ways(preserved at -20 ℃,preserved at 4 ℃,natural drying and silica gel drying) and extraction methods (SDS,CTAB,SDS-CTAB,and modified of CTAB). The results showed that -20 ℃ was a better way for sample preservation and the modified CTAB method was a better method for DNA extraction than others. The UV detection value of A260/A280 was 1.7-1.8 for DNA extracted by this modified CTAB method,obviously higher than that of others,such as 1.1-1.5 for SDS method,1.2-1.5 for CTAB method,1.4-1.6 for SDS-CTAB method. The agarose electrophoresis,restriction endonuclease digestion detection and PCR amplification also indicated that the modified of CTAB method was better than others.