四川大学学报(自然科学版)
四川大學學報(自然科學版)
사천대학학보(자연과학판)
JOURNAL OF SICHUAN UNIVERSITY
2009年
6期
1855-1861
,共7页
张建军%刘世贵%余懋群%敖清艳
張建軍%劉世貴%餘懋群%敖清豔
장건군%류세귀%여무군%오청염
冬瓜%农艺性状%隶属函数%分类%遗传多样性%RAPD
鼕瓜%農藝性狀%隸屬函數%分類%遺傳多樣性%RAPD
동과%농예성상%대속함수%분류%유전다양성%RAPD
Chinese wax gourd (Benincasa hispida Cogn.)%agronomic character%subordination function%classification%genetic diversity%RAPD
本文对我国100份冬瓜种质资源的熟性与果实特征进行详细的研究与分析,并利用隶属函数进行冬瓜的熟性、果形的分类;提出冬瓜的熟性、大小与果形分类的量化指标.对其中70份资源采用RAPD分子标记技术分析.挑选11条RAPD引物对这些资源进行遗传多样性分析.共扩出74 带,其中有多态性位点30个.这些冬瓜品种资源的相似系数在0.703~0.986之间.
本文對我國100份鼕瓜種質資源的熟性與果實特徵進行詳細的研究與分析,併利用隸屬函數進行鼕瓜的熟性、果形的分類;提齣鼕瓜的熟性、大小與果形分類的量化指標.對其中70份資源採用RAPD分子標記技術分析.挑選11條RAPD引物對這些資源進行遺傳多樣性分析.共擴齣74 帶,其中有多態性位點30箇.這些鼕瓜品種資源的相似繫數在0.703~0.986之間.
본문대아국100빈동과충질자원적숙성여과실특정진행상세적연구여분석,병이용대속함수진행동과적숙성、과형적분류;제출동과적숙성、대소여과형분류적양화지표.대기중70빈자원채용RAPD분자표기기술분석.도선11조RAPD인물대저사자원진행유전다양성분석.공확출74 대,기중유다태성위점30개.저사동과품충자원적상사계수재0.703~0.986지간.
The main agronomic characters were researched and analyzed in wax gourd including ripeness and fruit among 100 accessions from different region in China. Subordination function was used to classify them by main agronomic characters including ripeness and fruit. Quantitative classification indexes of ripeness and fruit shape in wax gourd were put forward. Random amplified polymorphic DNA (RAPD) molecular markers were used to detect the genetic diversity among 70 Chinese wax gourd germplasms.11 selected RAPD primers were used to analyze the diversity. A total of 74 DNA bands were amplified,30 of which were polymorphism. The genetic similarity among 70 Chinese wax gourds varied from 0.703 to 0.986.